摘要
目的:观察在大鼠肾移植模型中依达拉奉对移植术后24h移植肾的影响及其作用。方法:36只雄性SD大鼠,随机分为3组:假手术组(A组)、正常移植组(B组)、依达拉奉治疗移植组(C组),每组12只。B组为左肾原位移植,C组为移植术后予依达拉奉(10mg.kg-1)干预。观察移植术后24h各组血清肌酐、尿素氮、移植肾肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)及移植肾损伤程度。结果:与B组比较,C组血清肌酐水平明显下降(301.75±27.53比242.73±30.21,P<0.05);TNF-α转录水平(0.36±0.02比0.29±0.04,P<0.05)、表达水平(212.72±20.92比179.62±21.41,P<0.05)及IL-1β表达水平均明显下降(44.82±10.21比32.21±9.17,P<0.05),肾组织损伤程度明显减轻(2.68±0.13比2.02±0.21,P<0.05)。结论:依达拉奉能够抑制细胞因子TNF-α、IL-1β,改善大鼠同种异体移植肾功能。
Objective：To observe the role of edaravone on rat kidney allograft in rat kidney transplantation models after 24 hours. Methods： thirty six male SD rats were served as donors and recipients. Animals were randomly divided into the following three groups： the sham-operated group （A）, regular transplantation group （B）, and edaravone treatment group（C）. Group B was suffered normal renal transplantation and Edaravone（10 mg · kg-1 ） was delivered intravenously after transplantation in Group C. The changes of renal function and the degree of ischemic reperfusion injury of kidney graft combined with the expres- sions of TNF-α or IL-1β were observed 24 hours aher renal transplantation. Results： Compared with Group B, group C significantly decreased creatinine（301.75 ± 27.53 vs. 242.73± 30.21 ,P〈0.05） ; TNF-α transcriptional level（0.36 ± 0.02 vs. 0.29± 0.04,P〈0.05）, expression level （212.72 ± 20.92 vs. 179.62 ± 21.41 ,P〈0.05） and expression of IL-1β levels （44.82 ± 10.21 vs. 32.21 ± 9.17 P〈0.05）were decreased. The degree of injury in group C was significantly lower than that in the group B（2.68 ± 0.13 vs. 2.02 ± 0.21 ,P〈0. 05）. Conclusions： the inhibition of TNF-α and IL-1β by edaravone might play the role for rat kidney graft function protection.
出处
《中国临床医学》
2010年第1期98-100,共3页
Chinese Journal of Clinical Medicine
