曲古抑菌素A抑制乳腺癌MCF-7细胞增殖和诱导其凋亡的作用

Inhibitory And Apoptosis-inducing Effect of Trichostatin A on Human Breast Cancer Cell Line MCF-7

目的:研究组蛋白去乙酰化酶抑制剂曲古抑菌素A对乳腺癌MCF-7细胞的增殖抑制作用和诱导凋亡作用。方法:乳腺癌MCF-7细胞经不同剂量曲古抑菌素作用后,用二甲氧唑黄比色法(XTT)法测定曲古抑菌素A对乳腺癌MCF-7细胞的增殖抑制率;用免疫细胞化学染色法观察其对凋亡相关基因p21wafl表达的影响。结果:不同浓度的曲古抑菌素均可抑制MCF-7细胞的增殖,且抑制率具有剂量和时间依赖性;凋亡相关基因p21wafl在曲古抑菌素A作用细胞中的表达明显高于未进行处理的细胞。结论:曲古抑菌素可抑制体外培养的人乳腺癌(MCF-7)细胞生长,诱导癌细胞发生凋亡。

Objective：To investigate the effects of histone deacetylase inhibitor （trichostatin A） on cell growth inhibition and apoptosis in MCF-7 human breast cancer cell lines. Methods： The 2,3-his E2 methoxy 4-nitro-5 sulfophenyl, 2H-tetrazolium-5- carboxanilide （XTT） assay was used to observe the inhibitory complexion of trichostatin A on MCF-7 human breast cancer cells at various concentrations. The expression of apoptosis related gene protein p21wefl was detected by immunohistochemical stai- ning. Results： MCF-7 cells growth was significantly inhibited by sodium butyrate and the inhibitory affect was in time and dose- dependent manner;The level of p21wsfl was higher in the sodium butyrate treated cell than the cell without treatment. Conclusions： Trichostatin A can inhibit the cell growth and induce apoptosis in MCF-7 human breast cancer cell.