高胆固醇血症患者血浆对氧磷酯酶1活性变化的研究

Changes of Paraoxonase1 Activity in the Patients with Hypercholesterolemia

目的:观察高胆固醇血症(HC)患者血浆对氧磷酯酶1(PON1)活性的变化及其与高胆固醇血症之间的关系。方法:测定109例高胆固醇血症组患者和66例对照组患者血浆PON1活性、血脂、氧化修饰低密度脂蛋白(ox-LDL)水平及丙二醛(MDA)含量,并进行对比分析。结果:高胆固醇血症患者血浆PON1活性(138.1±62.4kU.L-1)较对照组(171.4±66.2kU.L-1)显著降低(P<0.01);而MDA及ox-LDL含量(12.8±3.8μmol.L-1,589.1±198.2μg.L-1)均较对照组(9.7±2.3μmol.L-1,455.1±178.2μg.L-1)显著升高(P<0.01)。相关分析显示,PON1活性与MDA及ox-LDL含量呈显著负相关(r=-0.441,P<0.01;r=-0.402,P<0.01)。结论:高胆固醇血症患者存在的氧化应激可能导致血浆PON1活性降低。

Objective： To investigate the changes of paraoxonase 1 （PON1） in the patients with hypercholesterolemia, and the relationship between paraoxonasel activity and hypercholesterolemia. Methods： The fasting plasma PON1 activity, oxidized low-density lipoprotein （ox-LDL） level and malondialdehyde （MDA） level and lipids levels in 66 normal controls and 109 hy- percholesterolemia patients were determined. Results： The PON1 activity in hypercholesterolemia （138.1 ± 62.4μu·L^-1 ） was significantly decreased as compared with normal controls （171.4 ± 66.2 μg·L^-1）（p〈0. 01）. The MDA level in hypercholes- terolemia （12.8 ± 3.8 μmol·L^-1 ） was significantly increased as compared with normal controls （9.7 ± 2.3μmol·L^-1 ） （p〈 0.01）, and so did the ox-LDL level （589.1 ± 198.2 μg·L^-1） vs （455.1 ± 178.2μg·L^-1 ）. Correlation analysis indicated that there was a negatively correlated with MDA （r = -0. 441, P〈0.01 ）, ox-LDL（r = -0. 402, P〈0.01 ）. Conclusions： Increased oxidative stress present in hypercholesterolemia may lead to decrease of plasma PON1 activity.