徐长卿子叶组织培养及再生体系的建立

Tissue Culture and Establishment of Regeneration System for Cynanchum paniculatum

以徐长卿子叶为材料,进行愈伤组织诱导、愈伤组织和不定芽分化、试管苗生根、移栽及移植等研究。结果表明,MS+6-BA0.3mg/L+NAA0.2mg/L+2,4-D0.4mg/L是子叶愈伤组织诱导和继代培养的理想培养基;MS+6-BA0.8mg/L+NAA0.2mg/L是愈伤组织分化培养的理想培养基;MS+6-BA0.6mg/L+NAA0.2mg/L是不定芽分化继代培养的理想培养基;炉灰渣是试管苗移栽的理想基质,移栽成活率可达97%。试管苗移植后长势旺盛,根系发达,当年开花。根据本试验结果可建立徐长卿子叶诱导再生体系。

The induction and differentiation were studied on the callus of cotyledon of Cynanchum paniculatum and rooting and transplanting of test-tube plantlets as well as establishment of asexual system of Cynanchum paniculatum were successfully carried out in the experiment. The results suggested： MS＋6-BA0.3 mg/L ＋NAA0.2 mg/L ＋2,4-D 0.4 mg/L was the optimum medium for callus induction and subculture of cotyledon. The ideal medium for callus differentiation was MS＋6-BA0.8 mg/L ＋NAA0.2 mg/L. The medium of MS＋6-BA0.6 mg/L ＋NAA0.2 mg/L was optimal for differentiation and subculture of adventitious buds. And the cider was used as ideal matrix for plantlets culture with vigorous and variant characters. Based on the result of tiffs experiment, induction and regeneration system of Cynanchum paniculatum could be set up.