摘要
目的:探讨P-gp、MRP1、BCRP等ABC转运蛋白在肝细胞癌中的表达及其与临床病理特征的关系。方法:应用RT-PCR和免疫组织化学方法检测P-gp/MDR1、MRP1、BCRP等mRNA及蛋白在34例的肝细胞癌、19例癌旁肝组织中的表达。结果:MDR1、MRP1、BCRP等mRNA在肝癌组的相对表达水平分别为1.15±0.24、0.64±0.33、1.07±0.32,在癌旁肝组织组的相对表达水平分别为0.36±0.14、0.19±0.06、0.31±0.09。MDR1、MRP1、BCRP在肝细胞癌中低分化组(Ⅲ、Ⅳ级)为1.38±0.26、0.73±0.35、1.34±0.21,在高分化组(Ⅰ、Ⅱ级)分别为0.74±0.32、0.30±0.11、0.45±0.13。P-gp、MRP1、BCRP等的阳性反应产物主要分布于细胞膜和胞浆内;在肝癌组和癌旁肝组织组的阳性表达率分别为82.35%、58.82%、79.4l%和42.11%、26.32%、36.84%。P-gp、MRP1、BCRP在肝细胞癌中低分化组(Ⅲ、Ⅳ级)的阳性表达率分别为100%、81.25%、100%,在高分化组(Ⅰ、Ⅱ级)的阳性表达率分别为66.67%、38.89%、61.11%。RT-PCR及免疫组织化学均显示,P-gp、MDR1、MRP1、BCRP mRNA和蛋白在肝癌组的表达均高于癌旁肝组织组,且在低分化(Ⅲ、Ⅳ级)肝癌组织的表达高于高分化者(Ⅰ、Ⅱ级),差异有统计学意义(P<0.05)。MDR1、MRP1、BCRP之间的表达无线性相关。结论:在肝细胞癌中存在原发性多药耐药现象,且多种耐药机制并存。P-gp、MDR1、MRP1、BCRP等与肝细胞癌的耐药有关,在肝癌组的表达高于癌旁肝组织组,且在肝细胞癌中的表达随着肿瘤分化程度的增高而降低,可作为肝细胞癌多药耐药性作用的靶点。
Objective: To investigate the expression of ATP-Binding Cassette Proteins including P-gp (P-glycoprotein), MRP1 (multidrug resistance associated protein 1) and BCRP (breast cancer resistance protein) in hepatocellular carcinoma and its relationship with pathological features. Methods: The expression of P-gp/MDR1 (multidrug resistance gene 1), MRP1 and BCRP in hepatocellular carcinoma was examined by RT-PCR and immunohistochemistry in 34 cases of hepatocellular carcinoma and 19 cases of paraneoplastic hepatic tissues. Results: The expression of MDR1, MRP1 and BCRP mRNA (messenger ribonucleic acid) was 1.15± 0.24, 0.64± 0.33, and 1.07 ± 0.32 in hepatocellular carcinoma and 0.36±0.14, 0.19±0.06, and 0.31±0.09 in paraneoplastic hepatic tissues. The expression of MDR1, MRP1 and BCRP mRNA was 1.38±0.26, 0.73±0.35, and 1.34±0.21 in poorly differentiated hepatocellular carcinoma and 0.74+0.32, 0.30±0.11, and 0.45±0.13 in well differentiated hepatic tissues. The immunohistochemical positive substance was detected in the plasma membrane and cytoplasm. The positive rates of P-gp, MRP1 and BCRP were 82.35%, 58.82%, and 79.41% in hepatocellular carcinoma and 42.11%, 26.32%, and 36.84% in paraneoplastic hepatic tissues, respectively. The positive rates of P-gp, MRP1 and BCRP were 100.00%, 81.25%, and 100.00% in poorly differentiated hepatocellular carcinoma and 66.67%, 38.89%, and 61.11% in well differentiated hepatic tissues. The expression of three indicies in hepatocellular carcinoma was higher than that in paraneoplastic hepatic tissues (P〈0.05). The expression of P-gp/MDR1, MRP1 and BCRP in poorly differentiated hepatocellular carcinoma was higher than that in well differentiated hepatic tissues (P〈0.05). No correlation was found among the three indices. Conclusion: Intrinsic multidrug resistance exsists in hepatocellular carcinoma, with various mechanisms. The multidrug resistance of HCC (hepatic cell carcinoma) is related to P-gp/MDR1,
MRP1 and BCRP. MRP1 and BCRP may be targets for reversing multidrug resistance.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2010年第4期190-193,共4页
Chinese Journal of Clinical Oncology
基金
山东省"泰山学者"建设工程专项经费
山东省科技攻关计划基金资助(编号:2006GG3202016)~~