细胞增殖微血管密度及临床病理学参数对滑膜肉瘤复发的影响

The Influence of Cell Proliferation and Microvessel Density on the Recurrence of Synovial Sarcoma

目的:探讨肿瘤细胞增殖、凋亡、微血管密度及临床病理学参数对滑膜肉瘤患者复发的预后意义。方法:选择2006年1月至2009年1月期间本院临床及随访资料完整并在随访期间未见转移的滑膜肉瘤56例,采用免疫组织化学染色检测Ki-67和CD31的表达,应用TUNEL染色检测肿瘤细胞凋亡情况,比较上述因素及临床病理学参数与复发的关系,并分析它们对患者无复发生存的影响。结果:1)在随访期间41例(73.2%)患者出现复发,中位无复发生存时间为19.5个月,患者术后1、2、3、4、5年的无复发生存率分别为45.0%、41.0%、34.0%、28.0%和28.0%。2)56例滑膜肉瘤中,Ki-67标记指数为19.98%±11.64%,微血管密度为51.83±21.92个/高倍视野(×400),凋亡指数为0～9.0%。3)组织学类型(P=0.000)和微血管密度分级(P=0.045)在复发与无复发组之间差异有统计学意义。4)单因素分析结果显示,Ki-67表达(P=0.009)、组织学类型(P=0.012)、放疗(P=0.014)与滑膜肉瘤患者的无复发生存有关。5)多因素分析结果表明,组织学类型(RR=0.207,P=0.031)、Ki-67表达(RR=1.944,P=0.045)和放疗(RR=0.482,P=0.04)是滑膜肉瘤复发的独立危险因素。结论:组织学类型和微血管密度可能影响滑膜肉瘤的复发,并且组织学类型和肿瘤细胞增殖情况可能影响滑膜肉瘤患者的无复发生存。

Objective： To study the prognostic significance of cell proliferation and apoptosis, MVD and clinicopathological parameters for the recurrence of synovial sarcoma. Methods： We analyzed the clinical and follow-up data of 56 synovial sarcoma patients without metastasis. RT-PCR was used to detect the subtype of SYT-SSX fusion gene. The expression of Ki67 and MVD was detected by immunohistochemistry. Univariate analysis was employed to analyze the influence of the above factors and clinicopathological parameters on the recurrence free survival and to explore the influencing factors for the recurrence of synovial sarcoma. Results： Of all the patients, 73.2% （41/56） had recurrence during the follow-up. The median recurrence free survival was 19.5 months. The recurrence free 1-, 2-, 3-, 4-, and 5-year survival rates after surgery were 45.0%, 41.0%, 34.0%, 28.0%, and 28.0%, respectively. Ki-67 labeling index （LI） was 19.98%±11.64% and MVD was 51.83±21.92 per ×400. There was no significant difference in apoptotic index （AI） between the two groups （P=0.607）. χ2 analysis showed that histological type （P=-0.000） and MVD （P=-0.045） were significantly correlated with the recurrence of sy- novial sarcoma. Univariate analysis showed that Ki67 LI （P=0.009）, histological type （P=-0.012） and radiotherapy （P=- 0.014） were significantly correlated with the recurrence free survival of synovial sarcoma patients. Sex （P=0.015）, tumor location （P=-0.411）, tumor size （P=-0.801）, necrosis （P=0.486）, MVD （P=-0.454）, chemotherapy （P=0.272）, and apoptotic grade （P=0.899） were not correlated with the recurrence free survival of synovial sarcoma patients. Multivariate analysis re- vealed that higher expression of Ki67 （RR=1.944, P=-0.045）, radiotherapy （RR=0.482, P=0.04）, and histological type （RR= 0.207, P=-0.031） were independent risk factors for the recurrence of synovial sarcoma. Conclusion： The expression of Ki67, radiotherapy and histological type are important factors for evaluating the recurrence and prognosis of synovial sarcoma.