内皮素和降钙素基因相关肽在咳嗽变异型哮喘患者中的相关性分析

Study of the correlation of ET and CGRP in cough variant asthma patients.

目的探讨血浆内皮素、降钙素基因相关肽（CGRP）在咳嗽变异型哮喘（CVA）患者血浆中的浓度、相关性及其临床意义。方法选择CVA患者30例（CVA组）、典型支气管哮喘急性发作期患者30例（典型哮喘组）和25名健康对照者（健康对照组）。对每例研究对象抽取清晨空腹静脉血3ml，用放射免疫法测定内皮素、CGRP，对相关数据进行统计学分析。结果CVA组、典型哮喘组、健康对照组血浆内皮素浓度分别为（103．58±28．66）、（129．37±27．28）、（72．63±21．52）ng／L，CVA组与典型哮喘组内皮素浓度显著高于对照组（P均〈0．01）；CVA组、典型哮喘组、对照组CGRP浓度分别为（7．62±2．56）、（6．63±2．09）、（21．60±3．29）ng／L，CVA组与典型哮喘组CGRP水平显著低于对照组（P均〈0．01）；CVA组与典型哮喘组内皮素、CGRP水平比较差异均无统计学意义（P均〉0．05）。CVA组血浆内皮素和CGRP水平呈显著负相关（r=-0．738，P〈0．05）；典型哮喘组血浆内皮素和CGRP水平呈显著负相关（r=-0．819，P〈0．05）。结论CVA患者与典型哮喘发作期患者一样，其血浆内皮素和CGRP水平呈显著负相关，提示内皮素、CGRP在CVA中是一对相互拮抗因素，内皮素水平升高、CGRP水平降低在CVA中起着重要的促发或加剧作用。

Objective To understand the levels of plasma endothelin （ET） and calcitonin gene-related peptide （CGRP） in cough variant asthma（CVA） patients, and to investigate their correlation and clinical implications. Methods Thirty CVA patients,30 typical bronchial asthma patients and 25 healthy controls were recruited. Three milliliter limosis venous blood were drawn from each patient to measure the levels of plasma ET and CGRP by radioimmunity. Results ①The levels of ET in the CVA group and typical bronchial asthma group were higher than that in healthy controls （ P 〈 0.01 ）. Their values were （ 103.58 ± 28.66 ） ng/L, （ 129.37 ± 27.28 ） ng/L and （72.63 ±21.52）ng/L, respectively. The levels of CGRP in the CVA group and typical bronchial asthma group were （7.62 ± 2.56）ng/L and（ 6.63 ± 2.09 ）ng/L. The level of CGRP in the healthy controls group was （21.60 ± 3.29 ） ng/L. The first two groups were lower than the latter（ P 〈 0.01 ）. However, there was no significant difference between the CVA group and typical bronchial asthma group（P 〉 0.05）. ②ET and CGRP were negatively correlated in both CVA group （ r = - 0.819, P 〈 0.05 ） and typical bronchial asthma group （ r = - 0. 738, P 〈 0.05 ）. Conclusions ET and CGRP were negatively correlated in both CVA group and typical bronchial asthma group, which means that ET and CGRP were a couple of antagonistic factors participated in the regulation of CVA, and may play an important role in the process of CVA.