大鼠急性脊髓损伤热休克蛋白70的表达

Expression of heat shock protein 70 in rats with acute spinal cord injury

目的:观察大鼠急性脊髓损伤后脊髓组织中热休克蛋70的表达。方法:Wistar大鼠56只,随机分成7组,6组制作成急性脊髓损伤模型,另一组作为对照组。分别于伤后2h、6h、12h、24h、48h、72h。建立脊髓损伤动物模型。应用West-blot方法观察各个时段损伤脊髓组织HSP70的表达。结果:急性脊髓损伤后2h,脊髓组织内出现HSP70的表达,损伤后24h脊髓组织中HSP70染色达到高峰,并可维持至损伤后72h。结论:在遭遇损伤性刺激后,脊髓组织HSP70的表达明显增加,表明HSP70有可能在阻止脊髓的继发性损伤方面发挥一定的作用。

Objective：To observe the expression of heat shock protein 70 （HSP70） in rats with acute spinal cord injury.Methods：Fifty-six Wistar rats were randomly divided into 7 groups.Six groups were prepared into acute spinal cord injury models and the other group was used as control.The animal models of spinal cord injury were established at 2h,6h,12h,24h,48h and 72h respectively after injury. The HSP 70 expression in the spinal cord from each group at different time points was studied by method of west-blot.Results：The expression of HSP70 was already found at 2h after the spinal cord injury, reached the peak at 24h and lasted to the time point of 72h after injury.Conclusion：The HSP70 expression is the response of the body to stress.HSP70 is obviously increased in the gray nucleus of the spinal cord from 2h to 72 h after acute injury.HSP 70 may play a role in the prevention of the spinal cord from secondary lesion.