CO_2气腹对胆囊癌细胞survivin表达的影响

Effect of CO_2 pneumoperitoneum on expressions of survivin in gallbladder carcinoma cell line

目的探讨CO2气腹对胆囊癌细胞抗凋亡能力的影响。方法建立腹腔镜CO2气腹体外模型,以12mmHg(1mmHg=0.133kPa)的CO2气腹气压对胆囊癌细胞进行不同时间(1,2,3,4h)的处理。设未处理为对照组。采用RT-PCR检测CO2气腹处理后各组细胞survivin mRNA的表达情况。结果当压力为12mmHg时,survivin的表达随气腹处理时间的延长而呈上升趋势(P<0.05)。结论在CO2气腹(12mmHg)下作用3～4h能提高胆囊癌细胞的抗凋亡能力。这可能是CO2气腹促肿瘤生长的机制之一。

Objective To study the effect of CO2 pneumoperitoneum on the anti-apoptotic capability of gallbladder carcinoma cell line （ GBC- SD ） . Methods The CO2 pneumoperitoneum was established in vitro. After GBC-SD cells were exposed to CO2 pneumoperitoneum at 12 mmHg for 1, 2, 3, 4 h, the expressions of survivin mRNA was examined by RT-PCR. The untreated GBC-SD cells served as control. Results Under a pressure of 12 mmHg, the expressions of survivn had an increasing tendency with the exposure time. Conclusions Under certain pressure and within certain exposure time, CO2 pneumoperitoneum could improve anti-apoptotic capability of GBC-SD cells, which may be one of the mechanisms of the promoting effect of carbon dioxide pneumoperitoneum on malignant cell growth.