艾滋病病毒感染对酶联免疫吸附试验试剂检测静脉吸毒人员丙型肝炎病毒抗体反应强度影响的探讨

Study of the impact of HIV infection on the reaction intensity of HCV antibody to ELISA reagents among intravenous drug users

目的探讨艾滋病病毒(HIV)感染对ELISA试剂检测静脉吸毒人员丙型肝炎病毒(HCV)抗体的影响,为HIV、HCV混合感染检测策略的提出和修改提供理论依据。方法按是否感染HIV分组对两种抗-HCVELISA试剂检测反应强度(S/CO)≥1的分布进行对比,采用SPSS 15.0软件分析。结果两组样本经两种试剂检测反应强度(S/CO)的构成差异有统计学意义(P<0.05),HIV阳性组HCV抗体检测低反应强度的构成高于HIV阴性组(P<0.05)。对科华试剂检测为低反应强度的标本,与用Ortho试剂检测结果的一致性在两组间差异有统计学意义(P<0.05)。结论是否感染HIV对科华HCV抗体ELISA试剂及Ortho HCV 3.0 ELISA试剂反应强度均有影响。HIV感染者可能出现HCV抗体ELISA检测不确定或假阴性反应,弱阳性反应标本可增加假阳性可能。

Objective To evaluate the impact of HIV infection on the reaction intensity of HCV antibody to ELISA reagents among intravenous drug users, providing evidence for the development and revision of the diagnostic workup for HIV and HCV co-infection. Methods The intravenous drug users were divided into two groups as whether or not they were infected with HIV. The distribution of the reaction intensity to KHB and Ortho reagents （ S/CO≥ 1 ） was compared between the two groups. The analysis was performed using SPSS 15.0. Results Statistical differences were present in the S/CO constituent of the reaction intensity using different reagents （ P 〈 0.05 ） ; higher proportion of low S/CO was observed in the HIV-positive group than in the HIV-negative group （ P 〈 0.05 ）. Statistical significant differences were also remarkable in the consistency between the KHB and Ortho reagents when imposed to low S/CO samples （ P 〈 0.05 ）. Conclusion The status of HIV infection exerts certain influence upon the S/CO of HCV antibody subject to both the KHB and Ortho ELISA reagents. Uncertainty or false negative reaction of HCV antibody may be present in people infected with HIV in ELISA detection, and potential false-positives may obscure weak positive samples.