红米色素着生位置及其基因定位

The Pigment Location and Gene Mapping of Red Rice

分别对红米和白米做了石蜡切片观察,其色素位于果皮和种皮中;并对红米水稻材料红宝石进行了遗传研究及基因定位。红宝石与白色水稻R272的杂交F1表现为红色,表明色素基因受显性基因控制;同时,其F2群体米色性状遗传分离规律符合3∶1的分离比例,表明红色米皮的性状受1对显性基因控制。利用R272/红宝石的F2群体和微卫星标记,将该基因定位在第7染色体上RM8006和RM21186两个标记之间,其遗传距离分别为4.0cM和2.1cM,在物理图上这两个标记的距离为1.7Mb,并将该基因初步命名为Red。

It was observed that the pigment was in the pericarp and seed coat with paraffin section. Genetic analysis and gene location were conducted on red rice. All grains of FI population derived from red rice and the white rice （R 272） were all red rice,and the segregation ratio of the F2 population was fitted to 3：1 ,indicated that the red pigment trait was controlled by a completely dominant gene. Using the F2 population from the cross between R 272 and red rice and simple sequence repeats markers, a completely dominant gene temporarily named Red,was located between RM 8006 （the genetic distance was 4.0 cM） and RM 21186 （the genetic distance was 2.1 cM） on the chromosome 7. The distance of two markers was 1.7 Mb on rice physical map.