连翘苷在大鼠小肠的吸收特性研究

The Intestinal Absorption Characteristics of Forsythin in Rats

目的研究连翘苷在大鼠小肠的吸收特性。方法采用外翻肠囊法建立连翘苷大鼠小肠吸收模型,HPLC测定连翘苷的含量,分别进行大鼠小肠的十二指肠、空肠、回肠3个肠段、不同浓度吸收特性研究,以及P-糖蛋白(P-gp)抑制剂盐酸维拉帕米和吸收促进剂吐温-80对连翘苷吸收的影响研究。结果连翘苷浓度为10,20,40μg.mL-1 3个浓度时,吸收速度常数ka无显著性差异(P>0.05);在不同肠段中的吸收,通过量由多到少依次为回肠>空肠>十二指肠;加入盐酸维拉帕米和吐温-80的小组与正常组相比,吸收速度常数ka无显著性差异(P>0.05)。结论在试验剂量范围内,连翘苷的吸收呈一级动力学过程,吸收机制主要为被动扩散;连翘苷不是P-糖蛋白的底物。

OBJECTIVE To study the intestinal absorption characteristics of forsythin in rats.METHODS Everted intestinal sac model was used and the concentration of forsythin was determined by HPLC.RESULTS There are no significant differences（P0.05） between ka of forsythin at three concentrations（10,20,and 40 μg.mL-1） of forsythin in perfusate;quantity of forsythin absorbed at small intestine in rats is ileumjejunumduodenum;verapamil hydrochloride and Tween-80 have no influence on absorption of forsythin.CONCLUSION The absorption of forsythin shows the first rate absorption characterize in the rat intestine,it is passive diffusion process,forsythin is not the substrate of P-gp.