正交试验法优选通便胶囊的提取工艺条件

Optimization of Extracting Process Conditions of Tongbian Capsule by Orthogonal Test

目的优选通便胶囊中木香、黄连、槟榔的提取工艺条件。方法结合中医理论,通过药效学试验验证,比较醇提物、水提物在小鼠肠推进方面的作用,确定提取工艺,并以干膏率、盐酸小檗碱的量为评价指标,采用L9(34)正交表对影响提取的因素醇浓度、用量、提取次数、时间进行考察。结果优选工艺条件为药材加10倍量30%乙醇,提取3次,每次1.5 h。结论经药效学实验验证、正交试验筛选得到的提取工艺条件科学合理可行。

OBJECTIVE To optimize the extraction process conditions from Costas,Coptis and Betel nut of Tongbian capsule.METHODS Combined of traditional Chinese medicine theory and passed pharmacodynamic study to compare ethanol extraction with water extraction to determine ethanolic route in the stimulative role of mice intestinal.Content of extraction and berberine hydrochloride are regarded as the assessing index to determine the optimum extracting conditions.Content of ethanol,amount of ethanol,extracting times and extracting time are selected by orthogonal design and the analysis of variance.RESULTS The optimum conditions of extraction process are 30% ethanol,which is 10 times of the amount of herb material,refluxed 3 times,1.5 h for each time.CONCLUSION The optimal extracting process conditions are scientific,reasonable and feasible by pharmacodynamic study and orthogonal test.