KMB17人胚肺二倍体成纤维细胞与多种肺癌细胞摄取^(99)Tc^m-MIBI的对比研究

^(99)Tc^m-MIBI uptake kinetic differences among KMB17 human embryonic lung diploid fibroblast and multiple human lung cancer cells

目的比较亲肿瘤显像剂99Tcm-甲氧基异丁基异腈(MIB)I在人胚肺二倍体成纤维细胞KMB17与多种肺癌细胞中的摄取情况,从细胞水平研究99Tcm-MIBI显像对肺肿瘤诊断和鉴别诊断价值的理论依据。方法在等体积且细胞浓度为1×106/mL的YTMLC个旧人肺鳞癌细胞、SPC-A1人肺腺癌细胞、AGZY低转移人肺腺癌细胞、973人高转移肺腺癌细胞、GLC-82个旧人肺腺癌细胞以及KMB17人胚肺二倍体成纤维细胞的混悬培养液中分别加入等量99Tcm-MIBI,按300μL精确取样分装于试管中,37℃恒温培养,均分别于5、15、30、45、60、75及90min时离心沉淀、测定细胞内放射性计数,计算各时间点各种细胞对99Tcm-MIBI的摄取百分率。结果6种细胞对99Tcm-MIBI摄取率有统计学差异,其摄取率大小关系为973>SPC-A1>GLC-82>AGZY>YTMLC>KMB17;30～45min时摄取率逐渐趋于平稳,45min摄取率均大于其摄取峰值的96.6%。结论99Tcm-MIBI对肺肿瘤的鉴别诊断有临床应用价值;且早期显像在30min左右是适合的。

Objective To evaluate the diagnostic and distinctive value for lung tumor with ^99Tc^m-MIBI through observing its uptake dynamic difference among human embryonic lung double-body fibrocyte KMB17 and several lung cancer cell lines. Methods Firstly, six different kinds of cell suspend culture liquid with equal cell density of 1 × 10^6/ml and same volume were prepared, which seperately contained YTMLC Gejiu human lung squamous carcinoma cells, SPC-A1 human lung adenocarcinoma cell, AGZY low metastatic human lung adenocarcinomas,973 high metastatic human lung adenocarcinoma cells,GLC-82 Gejiu human lung adenocarcinoma cell, and KMB17 human embryonic lung diploid fibroblast cells;secondly, the same radioactive dose of ^99^Tc^m-MIBI was added into each sample bottle and then, 300μl mixed liquid was taken out respectively and cultured intube and in37℃ ; Finally, 5,15,30,45,60,75 and 90 minutes after cultivation, centrifuged each cultured sample tube and determined the sediment＇s radiocounts of each sample, calculated each sample＇s uptake rate of ^99Tc^m-MIBI at different time. Results Statistical difference was found among six cell samples, and the uptake rate sequence from high to low is 973 〉 SPC-A 1 〉 GLC-82 〉 A GZY 〉 YTMLC 〉 KMB 17; furthermore, 30-45 minutes after culture, the uptake rate reached stability, and the 45 minutes uptake rate of each sample was higher than it＇s 96.6% uptake peak.Conclusion Based on the results above mentioned ,it is supposed that there are diagnostic and discriminative clinical value when use ^99Tc^m-MIBI as a tumor targeting imaging agent, and 30 minutes or so after injection may be the best imaging time in the early imaging stage.