肠球菌快速简易初筛方法的建立

Establishment of Rapid and Simple Primary Screening of Enterococcus

目的建立肠球菌属快速简易的初筛方法。方法采用七叶灵快速水解试剂盒,对139株革兰阳性球菌进行初筛,同时用常规方法及API 20Strep试条进行对比。结果肠球菌属荧光消失阳性率为99.26%,其中,粪肠球菌为100.00%、屎肠球菌为91.66%;浅灰色或黑色斑点形成阳性率为99.26%。结论利用七叶灵水解试剂盒、革兰染色、镜下形态及接触酶试验的结果,结合肠球菌在选择性鉴别培养基上的菌落特征,可快速简易鉴定肠球菌。

Objective To study and apply a rapid and simple screening method of Enterococcus.Methods A total of 139 strains of the gram-positive coccus were investigated for esculin hydrolysis by three methods of esculin rapid hydrolysis kit,conventional methods and API 20 Strep system（bioMerieux）.Results The positive rate of fluorescence loss was 99.26% in Enterococcus, 100% in Enterococcus faecalis,and 91.66% in Enterococcus faecium.The positive rate of forming light gray or black spots was 99.26%,of which Enterococcus faecalis was 100%,Enterococcus faecium for 91.66 %.Conclusion Through the methods of esculin hydrolysis,Gram＇s method,microscopic morphology,catalase test and colony characteristics on selective and differential medium,it can be rapidly and simply identified for Enterococcus at the genus level.