芸薹属SSR引物在甘蓝分子育种中通用性的研究

General Use of Brassica SSR Primers in Cabbage Molecular Breeding

利用SSR技术对甘蓝进行分子生物学研究,对影响其扩增效果的指标Mg^(2+)和dNTP进行筛选优化,获得了适宜甘蓝(C基因组)SSR的PCR条件。将已公布的在芸薹属不同作物上开发的78对引物运用于随机筛选的3株甘蓝材料上。结果表明:在78对芸薹属引物中,11对引物在3株甘蓝材料上表现良好的差异性,5对引物则表现无差异但是所得条带清晰。试验结果为进一步开展甘蓝分子育种奠定了基础。

During the study of molecular biology research in cabbage by using SSR technology, The first step was to explore much more SSR primers suitable to cabbage（C genome） and screen the optimized the PCR appropriate PCR conditions. In present study,we collected 78 pairs of SSR primers developed in different Brassica crops and explored the general use of these primer sets in cabbage with 3 random selected materials. Results showed lhat 11 among them had difference, and 5 pairs of primers showed no difference but had clear bands.