亮叶忍冬组织培养快繁技术

Studies on Tissue Culture and Rapid Propagation of Lonicera nitida‘Maigrun'

分别采用添加不同浓度6-BA的MS培养基和不含植物激素但改变大量元素浓度的MS培养基2种方法,研究亮叶忍冬组培苗快繁体系。结果表明:以不含激素的1/4MS培养基中亮叶忍冬的茎芽增殖效果最好,增殖系数达3.8。将亮叶忍冬试管苗茎段培养于用土做支撑物的培养基中,生根率为98.3%,根数也与琼脂支撑培养对照无明显差异,且根毛长于对照。对土支撑培养基中生根的亮叶忍冬试管苗开瓶练苗3周后,带坨移入营养钵中,在移栽后不喷雾、不覆膜,温室内午后空气相对湿度低至55%的条件下,移栽成活率达到93.3%,显著高于常规方法移栽的成活率(53.3%)。

This paper aims at setting up the system of tissue culture and rapid propagation technique of Lonicera nitida ＇ Maigrun＇. The MS media containing different concentration of 6-BA and the MS media with major element concentration changed without plant hormone were used respectively for the micro shut multiplication. The micro shuts multiplied best with the multiple coefficient of 3.8 on the 1/4 MS medium without plant hormones. The micro stem sections of Lonicera nitida ＇Maigrun＇ were cultured in soil supporting medium and 98. 3% of them rooted,the root number of which was no significant difference comparing with that of cultured in medium supported by agar,the root hairs of the plantlet from soil supporting medium were longer than that from agar supporting medium. After acclimatized in opened bottles for three weeks the rooted plantlets in soil supporting medium were transplanted into soil with lump and under the conditions of no-spraying, no-covering and low air humidity of 55 % in afternoon their survival rate was 93. 3 % which was significantly higher than that of the plantlet transplanted by normal method,the latter survival rate was 53. 3%.