HPCE法测定肉苁蓉中毛蕊花苷的含量

Determination of Acteoside in Herba cistanches by HPCE

建立了测定肉苁蓉中有效成分毛蕊花苷含量的高效毛细管电泳法。采用毛细管区带电泳（CZE）：运行缓冲液为30mmol/L硼酸．硼砂缓冲液（pH=9．00）；工作电压8kV；柱温25℃；检测波长：334nm。结果表明毛蕊花苷在0．014—0．112mg/mL的浓度范围内线性关系良好（r=0．9992），加样回收率100．1％，RSD=1．4％。结果显示高效毛细管电泳法用于测定肉苁蓉中毛蕊花苷的含量是可行的，本方法简便、快速、准确、重复性好。

To establish a HPCE method for determining actoeside in Herba cistanches, using the CZE to determine acteoside in Herba cistanches. The experimental condition was as follows ：30 mmol/L boron granule buffer（ pH = 9.0）, voltage 8 kV,temperature 25 ℃ and detective wavelength 334 nm. The linear range of of acteoside was 0.07-0.14 mg/mL（ r = 0. 9992） and the average recovery was 100. 1% （ RSD = 1.45% ）. The established method is simple, quick and accurate and suitable for determining acteoside in Herba cistanches.