摘要
以烟草烟雾水溶液（CSS）为材料，用MTT等方法研究CSS对人支气管上皮细胞（HBE细胞）的损伤及绿茶提取物对HBE细胞的保护作用及机理。结果表明：CSS抑制了HBE细胞活性，并呈良好的剂量关系；绿茶提取物在低浓度时（0～100μg／mL）对CSS引起的HBE氧化损伤有较好的保护作用，但在高浓度下（100～150μg／mL）促氧化作用将急剧上升，反而加剧了CSS所引起的氧化损伤。
This study was conducted to identify the protection of Green tea extract to the human bronchial epithelial cells damage by oxidant-stress. Cigarette smoke solution（CSS） were used to induce the renal damage of HBE cells in vitro. With the model of cell oxidant-stress, the inhibition effects of CSS on the growth of HBE cells were investigated by MTT assay. The results showed that CSS decreased the viability of HBE cells in a dose-dependent manner. Green tea extract significantly protect the viability of HBE cells induced by CSS in low concentration （0-100μg/mL）, but the oxidative stress will be promoted in high dose of Green tea extract（ 100-150 μg/mL） for human bronchial epithelial cells.
出处
《天然产物研究与开发》
CAS
CSCD
2009年第B05期216-219,共4页
Natural Product Research and Development
基金
基金项目：湖南省重大科技专项（2008FJ1005）
科技部支撑计划重点项目（2007BAD58B00-03）
关键词
人支气管上皮细胞
烟草烟雾水溶液
乳酸脱氢酶
丙二醛
活性氧
绿茶提取物
human bronchial epithelial cells （HBE）
cigarette smoke solution （ CSS ）
lactate dehydrogenase （ LDH ）
malondialdehyde （MDA）
reactive oxygen species （ROS）
tea extract
