姜黄素脂质体的制备及质量评价研究

Preparation research of curcumin liposome

研究姜黄素脂质体的制备方法。建立紫外可见分光光度法进行含量测定,采用薄膜分散法制备,均匀设计进行处方筛选,并以脂质体的包封率、渗漏率、形态、粒径分布等进行质量评价。薄膜分散法简单可行,筛选出的处方为胆固醇与磷脂1∶10,VE为0.045g,CUR为0.009g,Tween80为0.005g。经测定,包封率为74.47%,渗漏率为1.49%,姜黄素脂质体的平均粒径为166.83nm。紫外可见分光光度法适用于含量测定,薄膜分散高剪切法工艺合理易行,制得的脂质体包封率高,稳定性好,粒度均匀。

The research is mainly to study the processing techniques for prescription and preparation of curcumin liposomes. The UV method was set up to determine the encapsuled rate of CUR liposome. The liposome was prepared by thin-film disperse method. The prescription of liposomes was filtrated by uniform experiment. The quality was determined by encapsuled rate, leaky rate, et al. The preparation method was suitable and the prescription was CHOL ∶EPC =1 ∶10, VE 0.045 g, CUR 0.009 g, Tween 80 0.005 g. The encapsuled rate was 74.47%, leaky rate was 1.49%, size was 166.83 nm. The UV method was available for the determination of encapsuled rate. The preparation was simple and available for the high encapsuled rate, stable, well-proportioned of CUR liposome.