高效液相色谱法测定黑米提取物中矢车菊色素的成分及含量

Research on the content of the extract cyanidin(Cy)-3-glucoside in black rice by HPLC

利用高效液相色谱法测定黑米提取物的主要成分及含量。采用岛津VP-ODSC18柱(4.6mm×150mm)色谱柱,以磷酸(4%水溶液)∶乙腈(v∶v=8.8∶1.2,pH=2.0)为流动相,流速为0.8mL/min,柱温30℃,检测波长520nm。实验结果表明:该黑米提取物主要成分是矢车菊素-3-葡萄糖苷,平均含量为41.8479%。

We use the High Performance Liquid Chromatography method to measure the black rice＇s extract--major component and content of anthocyanin. We use shim-pack VP-ODS C18 （4.6 mm×150 mm）, and take phosphoric （4% water solution）： acetonitrile （v∶v=8.8∶1.2, pH=2.0） as mobile phase, set the velocity of flow at 0.8 mL/min, column temperature 30 ℃ and get the wave length 520 mm. So the result of the experiment＇s result shows that： the major component of anthocyanin in black rice＇s extract is Cy-3-glucoside which has the content of 41.8479%.