我国规模化肉牛场牛病毒性腹泻-粘膜病流行状况监测

Monitoring of Prevalence Status of BDV MD in Beef Cattle Herds in China

从山东、河南和河北省的６个规模化肉牛场随机采肉牛血清８９份，直肠粪拭子１６５份，分别用微量细胞中和试验和双抗体夹心ＥＬＩＳＡ试剂盒检测牛病毒性腹泻粘膜病（ＢＶＤＭＤ）的抗体和抗原。诊断结果：ＢＶＤＭＤ中和抗体阳性率为４６．２％～６５．０％；ＢＶＤＭＤ病毒抗原阳性检出率为５．１％～７７．２％。从而证实我国中原地区肉牛群中存在有ＢＶＤＭＤ。

Eighty-nine serum specimens and 165 rectum stool swabs were randomly sampled from bovine herds on 6 beef cattle farms in Shandong, Henan and Hebei provinces for examining the neutralization antibodies to bovine viral diarrhea mucosal disease (BVD MD) and the BVD MD virus antigens by means of a microdosage cell neutralization test and a double antibodies sandwich ELISA kit respectively. The diagnostic result revealed that the BVD MDV neutralization antibodies positive detectable rates ranged from 46.2 percent to 65.0 percent, and the BVD MDV antigens positive detectable rates were 5.4 percent to 77.2 percent, which confirmed that BVD MD has existed in beef cattle herds at the Zhongyuan regions, China.