摘要
从山东、河南和河北省的6个规模化肉牛场随机采肉牛血清89份,直肠粪拭子165份,分别用微量细胞中和试验和双抗体夹心ELISA试剂盒检测牛病毒性腹泻粘膜病(BVDMD)的抗体和抗原。诊断结果:BVDMD中和抗体阳性率为46.2%~65.0%;BVDMD病毒抗原阳性检出率为5.1%~77.2%。从而证实我国中原地区肉牛群中存在有BVDMD。
Eighty-nine serum specimens and 165 rectum stool swabs were randomly sampled from bovine herds on 6 beef cattle farms in Shandong, Henan and Hebei provinces for examining the neutralization antibodies to bovine viral diarrhea mucosal disease (BVD MD) and the BVD MD virus antigens by means of a microdosage cell neutralization test and a double antibodies sandwich ELISA kit respectively. The diagnostic result revealed that the BVD MDV neutralization antibodies positive detectable rates ranged from 46.2 percent to 65.0 percent, and the BVD MDV antigens positive detectable rates were 5.4 percent to 77.2 percent, which confirmed that BVD MD has existed in beef cattle herds at the Zhongyuan regions, China.
出处
《中国兽医科技》
CSCD
1998年第8期15-16,共2页
Chinese Journal of Veterinary Science and Technology
基金
"九五"国家重中之重项目
关键词
牛病毒性腹泻
粘膜病
监测
BVD MD monitor beef cattle Chinese library class number S 855.3