摘要
通过硫酸铵分级沉淀、DEAE-Sepharose Fast Flow阴离子交换柱层析、Sephacryl S-200分子筛柱层析3个步骤,从黑曲霉SL2-111发酵的果胶酶粗品中纯化出一种电泳纯的果胶酯酶。经SDS-PAGE测定其分子质量约为48.8ku。最适pH值和最适温度分别为5.8和60℃。在50℃以下,pH值5.8-6.2之间酶活力相对稳定。
A pectinesterase produced by Aspergillus niger SL2-111 was isolated and purified by three steps including ammonium sulfate precipitation, DEAE Sepharose Fast Flow ion exchang-e and Sephacryl S-200 filtration chromatography. The electrophoretically homogeneous pectineste-rase was obtained with its molecular weight estimated 48.8 ku on SDS-PAGE. Optimal pH and temperature were 5.8 and 60℃ , respectively. Its activity maintained relatively stable under such conditions as temperature below 50℃ and pH value between 5.8 - 6.2.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2010年第1期39-43,共5页
Food and Fermentation Industries
关键词
黑曲霉
果胶酯酶
分离纯化
酶学性质
Aspergillus niger, pectinesterase, purification, properties
