摘要
目的:研究P27和细胞周期素D1(CyclinD1)基因在喉各型组织尤其是不同分化程度喉鳞状细胞癌(喉鳞癌)中的表达,从而探讨其在临床诊断中的意义。方法:采用免疫组化SP法,检测P27、CyclinD1基因蛋白在正常喉黏膜、息肉、非典型增生及不同分化程度的喉鳞癌中的表达。结果:P27和CyclinD1均定位于细胞核。P27蛋白的表达随喉黏膜组织病变加重有逐渐降低的趋势,差异有统计学意义(P<0.01)。Cy-clinD1蛋白的表达随喉黏膜组织病变加重有逐渐升高的趋势,各组间差异有统计学意义(P<0.05)。结论:P27可作为判断喉鳞癌恶性程度的参考指标,而CyclinD1在喉鳞癌的发生、发展过程中是一个较早的分子事件,对喉鳞癌的发展起重要作用。
Objective：To study the expression and significances of the P27 and CyclinD1.Method：SP immunohistochemical method was used so to study the expression of P27 and CyclinD1 in laryngeal tissue esp,in laryngeal squamous carcinoma.Result：Immunoreaction of P27 and Cyclin D1 were located in the nuclei of cell.Positive expression of P27 was associated with histological grade（P0.01）.The expression of CyclinD1 were more and more stronger following with tissues being from benign to malignance.Conclusion：The P27 and CyclinD1 can be considered as the referential indicators for defining genesis of laryngeal cancer.P27 can be regarded as the indicator of considering tumor cell differentiation.CyclinD1 can be an early molecular event in the carcinogenesis of larynx and play an important role in the laryngeal carcingensis.
出处
《内蒙古医学杂志》
2010年第1期45-46,共2页
Inner Mongolia Medical Journal
