加味枳术汤对鼠肠缺血-再灌注早期小肠黏膜细胞凋亡的影响

The effects of Jiawei Zhishutang on the apoptosis of the intestinal mucosa in the rats with ischemia-reperfusion in the early stage

目的研究加味枳术汤对缺血-再灌注早期鼠小肠黏膜细胞凋亡的影响。方法将50只Wistar大鼠随机分为3组:对照组(n=10)、模型组(n=20)及治疗组(n=20);通过夹闭、开放肠系膜上动脉的方法建立小肠缺血-再灌注模型;治疗组术前灌胃给药(加味枳术汤);于术后1h、24h分别检测、对比3组小肠肠黏膜上皮Bcl-2、caspase-3及凋亡的变化。结果与模型组比较,治疗组的肠黏膜上皮细胞caspase-3的表达明显减低,Bcl-2的表达增强,上皮细胞凋亡指数明显降低。结论加味枳术汤可以明显改善缺血-再灌注早期肠黏膜细胞的凋亡,对小肠功能具有很好的保护作用。

Objective To study the effects of Jiawei Zhishutang on the apoptosis of the intestinal mucosa in the rats with ischemia-reperfusion（I/R） in the early stage. Methods Fifty Wistar rats were divided into three groups randomly： control group（n = 10）, I/R model group（n = 20） and treat- ment group （n=20）. The I/R model of the intestinal was established by blocking and opening the superior mesenteric artery. Jiawei Zhishutang was given by intragastric administration before the operation in the treatment group. At 1 st and 24 h after the operation, the changes of Bcl-2, caspase-3 and apoptosis of the epithelium mucosa were compared among the three groups. Results As compared the I/ R model group, the expression of caspase-3 and apoptosis index in the intestinal epithelium mucosa were obviously reduced, and the expression level of Bcl-2 in the intestinal epithelium mucosa was obviously increased in treatment group.Conclusion Jiawei Zhishutang can obviously alleviate apoptosis of the intestinal mucosa in the rats with I/R in the early stage,and can effectively protect the function of the small intestine.