马尾松雌雄球花的形成与赤霉素和脱落酸及细胞分裂素的关系

RELATIONSHIP BETWEEN THE FORMATION OF MALE OR FEMALE STROBILI AND THE LEVELS OF GA S,ABA,CTK S IN MASSON PINE

与其邻近的枝梢顶端相比，雌球花的赤霉素ＧＡ１＋３和ＧＡ４＋７的含量显著较高，而脱落酸（ＡＢＡ）含量并无明显差异；雄球花与其邻近的枝梢顶端相比，则ＡＢＡ含量显著较高，而两者的ＧＡ１＋３或ＧＡ４＋７含量差异不显著；不论是雌球花或雄球花，其细胞分裂素类中的一组，玉米素核苷组（ＺＲｓ）的含量均显著低于其邻近的枝梢顶端，而异戊烯腺嘌呤组（ｉＰＡｓ）或二氢玉米素核苷组（ＤＨＺＲｓ）的含量则未见显著差异。对４年生无花史的马尾松幼树施用了ＧＡ１＋３、ＧＡ４＋７、ＡＢＡ、萘乙酸（ＮＡＡ）等生长调节剂，发现ＡＢＡ可显著促进雄球花的形成，而ＧＡ１＋３、ＧＡ４＋７和ＮＡＡ则对雌、雄球花的形成均有促进效应。

The endogenous GAs (GA 1+3 ,GA 4+7 ),ABA and CTKs (iPAs, ZRs, DHZRs,)levels in male and female strobili and its nearby shoot apex of three types (Androdynamous, Gynodynamus and Sex-balanced) in masson pine were estimated with ELISAs. The results showed that there were higher levels of GAs in female strobili, and higher levels of ABA in male strobili, and much lower levels of ZRs in male or female strobili than in its nearby shoot apex. On the other hand, there was no clear difference between the GAs contents of male strobili or the ABA contents of female strobili respectively, in comparison with that in its nearby shoot apex. These indicated that female flowering was associated with higher ratio of GAs/ZRs and male flowering with higher ratio of ABA/ZRs. The experimental results of the chemical regulation indicated that ABA obviously enhanced the formation of male strobili, and GA 3,GA 4+7 and NAA significantly promoted the formation of female and male strobili.