摘要
目的对候选抑癌基因DOC-1R((Deleted in oral cancer-1 related,CDK2AP2)相关基因DCT1(DOC-1Rterminal1)进行染色体定位分析并观察其细胞内定位,检测该基因在人类组织中的表达。方法通过辐射杂交细胞系对其进行染色体定位分析,同时构建EGFP-DCT1质粒并转染HeLa细胞,荧光显微镜观察DCT1蛋白在细胞内的定位。此外,应用荧光定量PCR的方法检测该基因在16个正常人类组织中的表达。结果DCT1基因定位于5q31,其编码产物在HeLa细胞中定位于核膜,该基因在正常人类的16个组织中均有表达,其中在脾中表达水平较高。结论DCT1可能为细胞周期调控相关基因,在人类组织中普遍表达。
Objective To investigate the chromosomal and subcellular localization of DOC-1R teiminal 1 （ DCT1 ）,and detect its expression in human tissues. Methods Chromosome localization of DCTI was detected by radiation hybrid, pEGFP-DCT1 was constructed, and HeLa cells were transfected with the plasmid. The subcellular localization of DCT1 protein was observed by fluorescence microscope. Real-time PCR was performed for the determination of DCTI expression level in 16 kinds of human tissues. Results DCTI was demonstrated to localize in 5q31,and its encoding protein was detected on the nuclear membrane. Additionally,DCTl was proved to express universally in all the 16 kinds of human tissues and it was expressed at the highest level in spleen. Conclusion DCT1 might be a regulator in cell cycle,and ubiquitously express in human tissues.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2010年第2期105-107,111,共4页
Journal of China Medical University
基金
辽宁省教育厅重点实验室项目(20060908)
辽宁省教育厅创新团队项目(2008T194)
