Twist蛋白在非小细胞肺癌中的表达及临床意义

Expression and Clinical Significance of Twist Protein in Non-small Cell Lung Carcinoma

目的探讨扭曲蛋白(Twist protein)在非小细胞肺癌(NSCLC)中的表达及其临床意义。方法采用S-P免疫组化法检测61例有完整资料的NSCLC术后标本中Twist蛋白的表达情况。结果Twist蛋白在NSCLC组织中的阳性表达率明显高于正常肺组织;Twist蛋白的阳性表达与肿瘤组织类型、临床分期和淋巴结转移有关;对随访超过4年的48例患者的研究发现,生存时间<4年患者的Twist蛋白表达水平较高;术后死亡影响因子的Cox风险比例模型显示,吸烟及淋巴结转移对患者术后死亡率有显著影响。结论twist基因在NSCLC的发生发展中起一定的作用,并与患者的预后有一定关系。

Objective To study the clinical significance and the expression of Twist protein in non-small, cell lung cancer （NSCLC）. Methods The expression of Twist protein was examined in formalin-fixed paraffln-embedded tissue samples of 61 NSCLC by S-P immuno2 histochemical method. Results The expression level of Twist in NSCLC was significantly higher than that Of normal lung tissue; the positive expression of Twist protein was related to the histotype,clinieal stage and lymph node metastasis; the expression level of Twist protein in the cases whose survival time was less than 4 years was significantly higher than that in the cases whose survival time more than 4 years; the Cox analysis revealed that smoking and lymph node metastasis were the noticeable affeetive factors on the death of the NSCLC patients after surgery. Conclusion Twist gene played an important role in the genesis and development of NSCLC,and was related to the prognosis.