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结核杆菌抗原CFP21/MTB12/Rv3881c的HLA限制性细胞毒T淋巴细胞表位预测 被引量：4

Prediction of HLA restricted cytotoxic T lymphocyte epitopes in Mycobecterium tuberculosis CFP21/MTB12/Rv3881c
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摘要 目的:预测结核杆菌分泌抗原CFP21/MTB12/Rv3881c的HLA限制性细胞毒性T淋巴细胞(CTL)表位。方法:利用SYFPEITHI、BIMAS和NetCTL方法预测分析抗原CFP21/MTB12/Rv3881c的HLA-A*0201限制性CTL表位,进一步运用NetCTL方法对抗原CFP21/MTB12/Rv3881c的HLA-A其他等位基因和HLA-B限制性CTL表位进行预测分析。结果:初步筛选出3个候选抗原HLA-A*0201限制性CTL优势表位各4条,分别为CFP215-13、13-21、134-142、189-197;MTB1226-34、36-44、40-48、61-69;Rv3881c204-212、207-215、234-242、260-268。得到3个候选抗原HLA-A3和HLA-B7限制性CTL优势表位共21条。结论:初步筛选出抗原CFP21/MTB12/Rv3881c潜在的HLA-A*0201、HLA-A3、HLA-B7限制性表位33条。 Aim：To predict the HLA restricted cytotoxic T lymphocyte（CTL） epitopes in Mycobecterium tuberculosis CFP21/MTB12/Rv3881c.Methods：HLA-A0201 restricted CTL epitopes in CFP21/MTB12/Rv3881c were predicted by SYFPEITHI,BIMAS and NetCTL.The restricted T cell epitopes of HLA-B and the other alleles of HLA-A in CFP21/MTB12/Rv3881c were predicted by NetCTL.Results：Derived from the primary prediction results,the 12 predominant HLA-A0201 restricted T cell epitopes of the three candidates were CFP21 5-13,13-21,134-142,189-197,MTB12 26-34,36-44,40-48,61-69,and Rv3881c 204-212,207-215,234-242,260-268.And 21 predominant HLA-A3,B7 restricted T cell epitopes of the three candidates were also confirmed.Conclusion：Thirty three HLA-A0201,HLA-A3 and HLA-B7 restricted CTL epitopes derived from CFP21/MTB12/Rv3881c were predicted.
作者 吕虹 高艳锋 祁元明
机构地区 郑州大学生物工程系
出处 《郑州大学学报（医学版）》 CAS 北大核心 2010年第1期78-80,共3页 Journal of Zhengzhou University(Medical Sciences)
基金 河南省杰出人才基金资助项目084200510014
关键词 结核杆菌 CFP21 MTB12 Rv3881c 表位预测 Mycobecterium tuberculosis CFP21 MTB12 Rv3881 c epitope prediction
分类号 R52 [医药卫生—内科学]
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参考文献9

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共引文献6

同被引文献48

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二级引证文献5

郑州大学学报（医学版）
2010年 第1期

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