宫颈癌组织中Annexin A2和Ki-67蛋白的表达

Expression of Annexin A2 and Ki-67 in cervical cancer tissue

目的:探讨宫颈癌组织中AnnexinA2和细胞核增殖抗原(Ki-67)蛋白的表达。方法:采用免疫组化SP法检测74例宫颈癌组织、24例宫颈上皮内瘤变(CIN)组织及15例正常宫颈上皮组织中AnnexinA2和Ki-67蛋白的表达,并分析其与宫颈癌患者临床病理特征的关系。结果:①从正常宫颈组织→CIN组织→宫颈癌组织,AnnexinA2蛋白阳性表达率逐渐增高[(13.3%(2/15),37.5%(9/24)和71.6%(54/74)],差异有统计学意义(χ2=23.155,P<0.001);宫颈癌组织中其阳性表达率高于CIN组织和正常宫颈组织(P均<0.001),CIN组织与正常宫颈组织中其阳性表达率差异无统计学意义(P>0.05);AnnexinA2蛋白表达与宫颈癌患者的年龄(χ2=7.320,P=0.007)、组织类型(χ2=7.619,P=0.006)、病理分级(χ2=6.118,P=0.013)和淋巴结转移(P=0.015)有关。②从正常宫颈组织→CIN组织→宫颈癌组织,Ki-67蛋白阳性表达率逐渐增高[26.7%(4/15),37.5%(9/24)和82.4%(61/74)],差异有统计学意义(χ2=23.041,P<0.001);宫颈癌组织中Ki-67蛋白阳性表达表达率高于CIN组织和正常宫颈组织(P均<0.001),CIN组织与正常宫颈组织中其阳性表达率差异无统计学意义(P>0.05);Ki-67蛋白表达与宫颈癌患者的年龄(χ2=6.830,P=0.008)、病理分级(χ2=3.972,P=0.046)和临床分期(P=0.036)有关。结论:AnnexinA2和Ki-67可作为宫颈癌诊断的辅助指标,对评价宫颈组织的恶性程度及预后可能有一定的参考价值。

Aim：To explore the expression of Annexin A2 and Ki-67 in cervical cancer.Methods：The expressions of Annexin A2 and Ki-67 in 74 cases of cervical cancer,24 cases of CIN（Ⅰ～Ⅲ）,and 15 cases of normal cervical epithelium were examined by immunohistochemistry.Their relationship to clinicopathologic features of cervical cancer was analyzed.Results：①The positive rates of Annexin A2 expression in normal cervical epithelium,CIN and cervical cancer were 13.3%（2/15）,37.5%（9/24） and 71.6%（54/74）,respectively（χ2=23.155,P0.001）.The positive rate of Annexin A2 expression in cervical cancer was higher than that in CIN and normal cervical epithelium（P0.001）,and no statistical significance was found between normal cervical epithelium and CIN.Annexin A2 expression was related with the ages of cervical cancer patients（χ2=7.320,P=0.007）,histological substype（χ2=7.619,P=0.006）,pathological grade（χ2=6.118,P=0.013） and pelvic lymph node metastasis status（P=0.015）.②The positive rates of Ki-67 expression in normal cervical epithelium,CIN and cervical cancer were 26.7%,37.5%and 82.4%,respectively（χ2=23.041,P0.001）.The positive rate of Ki-67 expression in cervical cancer was higher than that in CIN and normal cervical epithelium（P0.001）.Ki-67 expression was related with the age of the cervical cancer patients（χ2=6.830,P=0.008）,pathological grade（χ2=3.972,P=0.046） and clinical stage（P=0.036）.Conclusion：Annexin A2 and Ki-67 may serve as a potential marker for cervical cancer diagnosis.It possibly has certain reference value in evaluating malignant degree of cervix organization and prognosis.