急性白血病患者骨髓单个核细胞中基质细胞衍生因子-1和血管内皮生长因子的表达

Expression of SDF-1 and VEGF in the bone marrow mononuclear cells of patients with acute leukemia

目的:探讨急性白血病(AL)患者骨髓单个核细胞中蛋白基质细胞衍生因子-1(SDF-1)及血管内皮生长因子(VEGF)的表达及其临床意义。方法:应用免疫组化SP法分别检测初治AL组(65例)、完全缓解期急性白血病(AL-CR)组(20例)、复发难治白血病(RRAL)组(24例)及正常对照组(15例)的骨髓单个核细胞中SDF-1及VEGF蛋白的表达情况,并分析SDF-1及VEGF蛋白的表达与初治AL患者临床特征的关系。结果:初治AL组骨髓单个核细胞中SDF-1及VEGF蛋白的阳性表达率分别为67.7%(44/65)和64.6%(42/65),均高于RRAL组[70.8%(17/24)和66.6%(16/24)]、AL-CR组[15.0%(3/20)和20.0%(4/20)]和正常对照组[6.6%(1/15)和13.3%(2/15)],差异有统计学意义(χ2=33.159和23.382,P<0.001)。骨髓单个核细胞中SDF-1和VEGF蛋白的表达与初治AL患者的白细胞计数(χ2=11.413和15.363,P<0.001)及髓外浸润(χ2=10.100和13.333,P<0.001)有关。结论:初治AL患者骨髓单个核细胞中SDF-1与VEGF蛋白的高表达参与了AL的发生、发展、浸润、复发和转移,动态检测2者的表达水平对判断AL的疗效和预后有重要意义。

Aim：To study the expression and clinical significance of stromal cell derived factor 1（SDF-1） and vascular endothelial growth factor（VEGF） in the bone marrow mononuclear cells of patients with acute leukemia（AL）.Methods：The expressions of SDF-1 and VEGF in the bone marrow mononuclear cells from untreated AL group（65 cases）,AL complete remission（AL-CR）group（20 cases）,relapsing,refractory AL（RRAL） group（24 cases）,and normal control group（15 cases） were detected by immunohistochemical SP assay.Results：The positive expression rates of SDF-1 and VEGF in untreated AL group [67.7%（44/65） and 64.6%（42/65）] were significantly higher than RRAL group[70.8%（17/24）and 66.6%（16/24）],AL-CR group [15.0%（3/20）and 20.0%（4/20）],and normal contral group[6.6%（1/15）and 13.3%（2/15）]（χ2=33.159,23.382,P0.001）.The expressions of SDF-1 and VEGF were related with the number of WBC in peripheral blood（χ2=11.413 and 15.363,P0.001） and leukemic infiltration（χ2=10.100 and 13.333,P0.001）.Conclusion：The overexpression of SDF-1 and VEGF in AL may play an important role in the pathogenesis,progression,infiltration,relapse and metastasis.It is contributed to judge prognosis and curative effect of AL by testing the change of expression of SDF-1 and VEGF dynamically.