急性黄磷及其化合物吸入致ALI/ARDS大鼠HSP_(70)的表达及肺组织病理改变

The changes of the expressions of heat stress protein-70 and pathology in lung tissues of ali/ards induced by aspirating yellow phosphorus and its compounds in rats

目的了解大鼠热休克蛋白70(HSP70)的表达及肺组织病理改变与急性黄磷及其化合物吸入致急性肺损伤/急性呼吸窘迫综合征(ALI/ARDS)的关系。方法健康SD大鼠48只随机分为对照组以及造模后0、4、12、24、48h处死组,每组8只。采用自制的气体发生以及染毒装置,将大鼠置入染毒柜后,间歇染毒形成ALI/ARDS模型。观察ALI/ARDS大鼠动脉血气分析变化和肺系数,肺组织HSP70的表达以及肺组织病理改变。结果肺损伤后大鼠动脉血气以及肺组织病理改变明显恶化,肺系数较对照组明显增大,正常大鼠肺组织HSP70表达较弱,表达部位为肺泡上皮和部分支气管黏膜上皮细胞胞质;在ALI/ARDS条件下,大鼠肺组织HSP70表达逐渐增强,至成模后12h时相点,HSP70的表达最强,并呈现明显核表达;至成模后48h时相点,HSP70的表达逐渐减弱,核表达逐渐消失,主要在肺泡上皮和部分支气管黏膜上皮细胞胞质表达。结论急性黄磷及其化合物吸入致ALI/ARDS大鼠肺组织病理改变随造模后时相的延长越来越严重。ALI/ARDS条件下大鼠肺组织HSP70表达表现为:受到应激-高表达-表达减弱,并伴有HSP70的再分布。

Objective To study the relationship between the expressions of heat stress proteins 70（HSP70） and the changes of pathology induced by aspirating yellow phosphorus and its compounds in rats.Methods 48 healthy SD rats were divided randomly into a matched control and build the model of ALI/ARDS made in 5 groups according to different hours（0,4,12,24 and 48 h respectively）.Placing the rat into a poisonous cabinet,intermittent dye poison formation ALI/ARDS model.Observe the arterial blood gas analysis and the lung coefficient,,the expressions of HSP70 and pathology of lung organization in rats.Result The changes of pathology and the arterial blood gas analysis became worsen after stimulation.The lung coefficient compared with the matched control enlarge obviously,In the normal control group,the expression of HSP70 in rats＇ lungs was not intensive,which could be only seen in cytoplasm of pulmonary alveolus epithelium cells and bronchial epithelium mucosa cells.ALI/ARDS increased HSP70 level,and the total content of HSP70 came to a peak level at 12 h after stimulation,while apparent expression of HSP70 in nucleus could be seen.At 48 h after stimulation,the content of HSP70 decreased and its expression in nucleus could not be seen.Conclusion The content of the changes of pathology increased with the phase after stimulation induced by aspirating yellow phosphorus and its compounds in rats.The expressions of HSP70 in ALI/ARDS performance is：stress reaction-high expression-low expression,with the redistribution of HSP70.