一株生淀粉酶产生菌的分离鉴定及酶学性质研究

Screening and identification of a fungus producing raw-starch amylase with new enzymatic properties

采用苔酚蓝-生淀粉法从贵州遵义百年磨坊的磨盘下的土样中筛选得到一株具有生淀粉降解能力的丝状真菌,经形态学鉴定和ITS核苷酸序列比对确定该菌为Rhizopus microsporusvar.chinensis。本文首次报道了由Rhizopus microsporus var.chinensis产生的生淀粉酶。通过酶学性质研究发现,该菌株所产生淀粉酶拥有较好的温度耐受性和较宽的pH适用范围,具有一定的工业应用价值。

A newly isolated producer of raw starch digesting amylase, Rhizopus microsporus vat. chinensis was screened and identified by morphological characteristics and molecular phylogenetic analysis. For the first time, raw starch digesting amylase produced by fungus of Rhizopus microsporus vat. chinensis was reported. Further studies found that the raw starch digesting amylase secreted by this strain performed a favorable thermostabilization and broad pH available range, which was valuable for industrial applications.