四物汤对血虚证模型大鼠肝线粒体蛋白质组的影响

Effect of Siwu Decoction on Liver Mitochondrial Proteome of Blood Deficiency Model Rats

目的探讨四物汤治疗血虚证的机理。方法Wistar大鼠随机分为模型组、治疗组、正常组各6只。模型组和治疗组大鼠在第1、4、7d皮下注射2%乙酰苯肼生理盐水注射液,第4d起模型组生理盐水灌胃,治疗组给予四物汤灌胃,正常组给予生理盐水,连用7d后,检测外周血象、肝脾湿重;提取肝脏线粒体蛋白质,用双向电泳等蛋白质组学技术测定大鼠肝线粒体中有影响的蛋白质。结果模型组大鼠出现萎靡、苍白等血虚症状,肝脾明显肿大,红细胞数和血红蛋白量明显降低,白细胞、网织红细胞计数明显升高(P<0.01);四物汤治疗后外观较快恢复,红细胞、血红蛋白、网织红细胞数较上升(P<0.01);模型大鼠肝线粒体蛋白质与正常相比有14个能量代谢相关蛋白质的水平发生改变,四物汤可使这些改变的蛋白质水平有所恢复。结论四物汤可以调节肝线粒体内一些酶和伴侣分子,清除自由基和过氧化物,稳定线粒体结构,保护线粒体功能;改善糖、脂类、蛋白质代谢,从蛋白质分子水平改善血虚动物的虚损状况。

Objective To observe the mechanism of Siwu Decoction （Decoction of four ingredients） on blood deficiency syndrome. Methods Wistar rats were randomized into 3 groups： model group, treatment group, and normal group, 6 ones in each. Rats of the model group and the treatment group were injected 2% acetylphenylhydrazine （APH） saline subcutaneously on the first, forth and seventh day. From the forth day, the model group was treated with saline by gavage, the treatment group was treated with Siwu Decoction by gavage, and the normal group was treated with saline for 7 days in succession. Then the amount of peripheral blood cells, wet weight of liver and spleen were detected. Liver mitochondrial proteome of all rats were extracted. Proteomic technology was used to measure the regulated proteins by Siwu Decoction. Results In the model group, the rats had decreased general status significantly such as pale, enlarged liver and spleen, lowered red blood cells （RBC） and hemoglobin （HGB）, increased white blood ceils （WBC）and retieulocyte （RCT） （P〈0.01）, and these changes were recovered quickly after treatment with Siwu Decoction, the 14 changed proteins related to energy metabolism were improved to a certain degree after treatment. Conclusion Siwu Decoction can improve the blood deficiency status at molecular level through regulating liver mitochondria, removing the free radicals and peroxides, stabilizing the mitochondrial structure, preserving mitochondrial functions, and improving carbohydrate, fat and protein metabolism.