塞来昔布对人结肠癌细胞VEGF-C表达的影响

Effect of Celecoxib on VEGF-C Expression in Colon Cancer Cell Line

[目的]探讨塞来昔布对体外培养的结肠癌细胞血管生长因子C(VEGF-C)表达的影响。[方法]以人结肠癌细胞HT29为研究对象,以不同浓度的塞来昔布(0、25、50、100μmol/L)进行处理。采用RT-PCR等方法检测四组细胞中VEGF-C的表达情况。[结果]塞来昔布可抑制人结肠癌HT29细胞VEGF-C的表达,抑制作用与塞来昔布的浓度相关。[结论]塞来昔布可能通过对肿瘤细胞中VEGF-C基因表达的抑制发挥其抗肿瘤作用。

[Purpose] To investigate the effect of celecoxib on VEGF-C expression in human colon cancer cell.[Methods] The colon cancer HT29 cells were treated with celecoxib at different concentrations 0μmol/L,25μmol/L,50μmol/L,100μmol/L.The expression of VEGF-C was detected by RT-PCR etc.[Results] Celecoxib could inhibit expression of VEGF-C in colon cancer cell HT29.The inhibition effect was correlated with the concentration of celecoxib.[Conclusion] Celecoxib might play antitumor role by inhibiting the expression of VEGF-C in colon cancer.