经前舒颗粒对经前期综合征肝气郁证大鼠下丘脑和海马雌激素受体α,β mRNA表达的影响

Effects of Jingqianshu granule on expression of estrogen receptor α and β mRNA in hypothalamus and hippocampus of PMS rats with Liver-qi depression

目的:研究中药复方经前舒颗粒对经前期综合征(PMS)肝气郁证模型大鼠下丘脑和海马中雌激素受体α(ERα)和β(ERβ)mRNA表达的影响。方法:以情志刺激多因素法复制PMS肝气郁证大鼠模型,使用中药复方经前舒颗粒进行干预治疗,采用RT-PCR法检测大鼠下丘脑和海马中ERα和ERβ mRNA表达变化。结果:与正常组大鼠相比,模型组大鼠下丘脑和海马中ERα和ERβ mRNA表达量高于正常组(P<0.05);与模型组大鼠相比,经前舒颗粒给药组大鼠下丘脑和海马中ERα和ERβ mRNA表达量则显著降低(P<0.05),与正常组无差异。结论:经前舒颗粒可下调下丘脑和海马中ERα和ERβ mRNA表达水平,这可能是其治疗PMS肝气郁证的作用机制之一。

Objective： To investigate the effects of Jingqianshu granule（JQS）,a Chinese medicinal formula on the expression levels of ERα and ERβ mRNA in hypothalamus and hippocampus of PMS model rats with Liver-qi depression.Method： The female SD rats were divided randomly into three groups： control,model and model plus Jingqianshu granule.We make the model rats of PMS with Liver-qi depression using the method of emotional stimulation multiple factors.Then we applied semi-quantitative RT-PCR gene amplification technology to detect the expression levels of ERα and ERβ mRNA in the brain regions of all rats.Result： Compared with the control group,the expression levels of ERα and ERβ mRNA in hypothalamus and hippocampus of model rats were higher significantly（P〈0.05）,while in the JQS-administration group,the expression levels of ERα and ERβ mRNA were lower than model group（P〈0.05）,and were no difference with the control group.Conclusion： The JQS granule can down-regulate the expression levels of ERα and ERβ mRNA in hypothalamus and hippocampus,which maybe one of the mechanism to treat PMS with Liver-qi depression.