硒化壳聚糖对HL60细胞的诱导分化作用

Inducing Differentiation Effect of Selenium Chitosan on HL60 Cells

目的:研究硒化壳聚糖对人早幼粒细胞性白血病HL60细胞分化的影响,并探讨该影响与细胞c-myc和c-fos蛋白表达的关系。方法:取HL60细胞分为5组,分别为25、50、100mg·L-1硒化壳聚糖组、空白对照组(培养液)和阳性对照组(二甲基亚砜)。各组经相应试药作用后,采用硝基蓝四氮唑(NBT)还原法检测HL60细胞分化情况,观察NBT阳性细胞数;流式细胞术检测c-myc和c-fos蛋白表达。结果:与空白对照组比较,硒化壳聚糖各组作用与阳性对照组相似,NBT还原阳性细胞明显增多,c-myc蛋白表达显著下降(P<0.01或P<0.05),c-fos蛋白表达显著升高(P<0.01或P<0.05)。结论:硒化壳聚糖可能通过增强HL60细胞c-fos表达及下调c-myc表达来诱导细胞分化。

OBJECTIVE： To study the effect of selenium chitosan on differentiation of HL60 cells and its relationship with the c-myc and c-fos protein expression of cells. METHODS： HL60 cells were divided into 5 groups, i.e. 25mg·L^-1 selenium chitosan group, 50 mg·L^-1 selenium chitosan group, 100 mg.L^-1 selenium chitosan group, blank control group and positive control group （dimethy sulfoxide）. The differentiation of HL60 cells was measured by NBT-reduced method after administration and amount of positive cells was detected. FCM was used to examine the c-myc and c-los protein expression of cells. RESULTS： As compared with blank control group, differentiation effect of selenium chitosan groups were similar to that of positive control group; NBT-reduced positive cells were increased while c-myc protein expression of cells was decreased （P〈0.01 or P〈0.05） ; c-fos protein expression of cells was markedly increased （P〈0.01 or P〈0.05）. CONCLUSION： Selenium chitosan could promote differentiation of HL60 cells by increasing the c-fos protein expression cells and decreasing c-myc protein expression.