土霉素片溶出度测定方法研究

Study on Determination Methods for Dissolution of Oxytetracycline Tablets

目的:建立土霉素片溶出度的测定方法。方法:在检测波长271nm下用紫外分光光度法测定土霉素含量;以0.01mol·L-1盐酸溶液作溶剂,选用桨法测定土霉素片溶出度。结果:土霉素检测浓度线性范围为4～32IU·mL-(1r=0.9999)。平均回收率为100.1%(RSD=0.65%);45min时样品溶出度均在75%以上。结论:所建立方法可用于土霉素片溶出度测定。

OBJECTIVE： To establish a determination method for dissolution of oxytetracycline tablets. METHODS： The content of oxytetracycline was determined by UV spectrophotometry at detection wavelength of 271 nm. Dissolution of oxytetracycline tablets was detected by slurry process using 0.01 mol·L^-1 hydrochloric solution as solvent. RESULTS： The linear range of oxytetracycline was 4-32 IU·mL^-1（r=0.999 9） with an average recovery of 100.1% （RSD=-0.65%）. The dissolution in 45 min were all above 75%. CONCLUSION： Established method in this study can be used to determine dissolution of oxytetracycline tablets.