小麦条锈菌水源11类群的RAPD分析及SCAR标记的建立

RAPD analysis and SCAR marker establishment of Su11 group of Puccinia striiformis f.sp.tritici in China

鉴于水源11类群近年来一直处于优势地位,为简化其检测手段,本研究利用RAPD技术对该类群的8个主要致病类型进行了多态性分析,以寻找其中主要流行类型的特异性分子标记。结果如下:共筛选出10个碱基随机引物190条,其中94条可得到稳定清晰的扩增图谱,用该94条引物进行RAPD分析,发现各致病类型间遗传变异丰富;以引物S1410扩增得到了水源11-4的特异性DNA条带;以引物S1412和S1304扩增得到了水源11-14的特异性DNA条带;对引物S1304扩增得到的特异性DNA条带回收、克隆和测序,设计了1对19bp/18bp的引物,并成功地将其转化为对水源11-14特异的SCAR标记。以上结果表明,通过规模筛选来寻找小麦条锈菌生理小种的特异性DNA片段,并将其转化为稳定的SCAR标记,有可能建立起中国小麦条锈菌流行生理小种的快速分子鉴定体系。

Suwonll（Su11） group has been becoming dominant in recent years. For simplification of identifying method, random amplified polymorphic DNA（RAPD） was used to analyze polymorphisms and find specific DNA band of eight pathotypes in the group. Of the 190 random primers analyzed in total, 94 primers firmly showed amplification profiles. The results indicated that the genetic variations among types of Su11 were abundance. The specific DNA fragment of Su11-4 was found by amplifying with primer S1410 and those of Su11-14 were found by amplifying with S1412 and S1304. The specific DNA fragment obtained with S1304 was cloned and sequenced. Based on the sequencing, a primer pair was designed and the SCAR marker for Su11-14 was obtained. The results indicated that a molecular identification system for Puccinia striiformis f.sp. tritici races could be established by means of searching specific RAPD fragments of different races.