慢性阻塞性肺疾病患者外周血淋巴细胞DNA的损伤及意义

Significance of DNA damage in peripheral blood lymphocytes of patients with chronic obstructive pulmonary disease

目的探讨慢性阻塞性肺疾病（chronic obstructive pulmonary disease，COPD）患者DNA的损伤及意义。方法采用胞质分裂阻滞微核分析法检测30例COPD患者及24名健康对照者外周血淋巴细胞的微核、染色体桥、核芽，以反映COPD患者DNA的损伤及与疾病严重程度之间的关系。结果微核率、染色体桥率、核芽率在COPD患者组的表达率分别为（61．12±14．05）‰、（12．77±6．82）‰、（11．21±5．27）‰，在健康对照组中分别为（2．98±5．21）‰、（4．85±3．29）‰、（3．56±2．52）‰，差异有统计学意义（P〈0．01）。微核率、染色体桥率、核芽率在COPD患者中度组、重度组、极重度组之间的差异有统计学意义（P值〈0．05），且COPD患者的微核率和染色体桥率与第1秒用力呼气容积占预计值百分比的改变均呈负相关（rs=0．0401，P=0．028；rs=-0．549，P=0．002）。结论COPD患者存在外周血淋巴细胞DNA的损伤，且DNA损伤的程度可能与疾病的严重度相关。

Objective To explore the levels of DNA damage in peripheral blood lymphocytes and its correlation analysis with the chronic obstructive pulmonary disease （COPD）. Methods The levels of genetic damage in peripheral blood lymphocytes from 30 patients with COPD and 24 healthy controls was evaluated by cytokinesis-blocked micronucleus （CBMB） methods, the frequencies of micronuclei （ MN）, dicentric bridge（DB）,nuclear buds（BUD）were used to investigate the DNA damage. Results Compared with control subjects, mean frequencies of MN, DB and BUD for patients with COPD showed an statistically significant（ P 〈0.01）. The frequencies of MN, DB and BUD were compared for patients in groups according to COPD severity as defined by GOLD, the moderate, severe, very severe showed differ significantly（all P〈0.05）. In patients with COPD, there was negative correlation between MN, DB and degree of airflow obstruction by FEV1% predicted（ r s=-0.040 1, P =0.028; r s=-0.549, P = 0. 002）. The level of showed a strongly negative correlation with DNA damage. Conclusions The result of CBMB in our study indicate an increased level of DNA damage in patients with COPD,the levels of DNA damage may be have a correlation with COPD severity.