原位转导胞嘧啶脱氨酶自杀基因对大肠肿瘤的杀伤作用

Study on the effect of suicidal gene of cytosine deaminase on colorectal carcioma

目的通过胞嘧啶脱氨酶(CD)基因对接种于裸鼠肿瘤的直接杀伤作用研究,探索原位转自杀基因抑制大肠肿瘤的治疗方法。方法将含 CD 基因的逆转录病毒载体进行包装、收获病毒上清后,将含 CD 基因的重组病毒上清直接注射到患瘤部位。结果在给予前药5-FC 后,观察到明显的肿瘤杀伤作用。结论 CD 基因直接转导到肿瘤部位的治疗结果,提示其可以做为大肠癌综合治疗的有效手段之一。

Objective To investigate the effect of cytosine deaminase(CD)/ 5-FC system in inhibiting growth of LoVo colorectal carcinoma grated in nude mice.Method The retroviral vector pCD2 containing CD suicidal gene was packaged in PA317 cells,the supernatant of recombinant retrovirus was then injected into tumor sites.Result Obvious regression of the tumor occurred after serial intraperitoneal administration of the prodrug for the 5-FC.Conclusion CD gene can be a useful tatic in the comprehensive treatment of colorectal carcinomas.