胸腺内注射Fas抗体对心脏移植大鼠Fas/FasL表达的影响

Influence of intrathymic injection of anti-Fas monoclonal antibody on Fas/FasL after heart transplantation

目的:研究受体胸腺内注射Fas抗体对心脏移植大鼠Fas/FasL表达的影响。方法:应用大鼠同种异位腹腔心脏移植模型,将模型动物随机分为三组,每组8只动物。A组:移植前后不给予处置;B组:给环孢素A,按每日8mg/kg于术前1d口服;C组:胸腺注射Fas抗体,以0.1mg/kg剂量在术前注射。以免疫组化法和逆转聚合酶链式反应(RT-PCR)法观察术后移植心脏局部的Fas及FasL的表达情况。结果:与A组相比,受体胸腺内注射Fas抗体可减少移植心脏局部的Fas/FasL基因表达(RT-PCR法半定量结果P<0.05)和蛋白表达(免疫组化法P<0.05)。结论:受体胸腺内注射Fas抗体是一种有效的抑制心脏移植急性排斥反应的方法,可使移植心肌局部Fas/FasL表达下调。

Objective：To investigate the influence of intrathymic injection of anti-Fas monoclonal antibody on Fas/FasL of rats after heart transplantation.Methods：Using rat allograft heterotopic heart transplantation models,rats were randomly divided into three groups：group A（control group）,group B（was treated with cyclosporin before test）,and group C（was treated with intrathymic injection of anti-Fas monoclonal antibody before test）. The number of rats of every group was eight.After operations,the samples were observed the express of Fas/FasL on the transplanted heart by RT-PCR and immunohistochemical method.Results：Compared to group A,intrathymic injection of anti-Fas monoclonal antibody significantly decreased the gene express （RT-PCR,semiquantitative） and protein level （immunohistochemistry） of Fas/FasL on the transplanted heart （P〈0.05）.Conclusion：Intrathymic injection of anti-Fas monoclonal antibody has a immunosuppressive effect on acute reject reaction and down regulation on the express level of Fas/FasL after rat heart transplantation.