氧化节杆菌与苯并[a]芘-镉交互作用机理

Interaction mechanism between Arthrobacter oxydans and BaP-Cd

利用HPLC、FTIR分析技术,研究了氧化节杆菌(Arthrobacter oxydans)对水体中苯并[a]芘(BaP)-镉(Cd)复合污染物交互作用机理。结果表明,BaP、Cd及BaP-Cd复合污染均对菌株的正常生长产生影响,其影响程度为:BaP-Cd>Cd>BaP;使用该菌投加到BaP、Cd浓度均为1mg·L-1的BaP-Cd复合污染无机盐溶液中,30℃、130r·min-1,培养5d后,体系BaP、Cd的残留量分别为0.39、0.65mg·L-1;添加一定浓度的H2O2并保持体系呈偏碱性更适于该复合污染的处理。红外扫描分析显示,在微生物作用下,BaP的结构发生改变,菌株降解BaP和吸附Cd的过程主要与羟基、CC键、酰胺基团和C—H键有关。

The interaction mechanism of benzo[a]pyrene（BaP）-cadmium（Cd）combined pollution in water body with Arthrobacter oxydans was investigated by using HPLC and FTIR analytical techniques.The experimental results showed that BaP,Cd and BaP-Cd combined pollution all posed adverse effects on the normal growth of the strain with the sequence of influence BaP-CdCdBaP.The concentrations of BaP and Cd in aqueous media were reduced to 0.39 mg·L-1 and 0.65 mg·L-1,respectively,from the initial concentration of 1 mg·L-1 after treatment for 5 days at 30℃,130 r·min-1.Adding H2O2 at a specific concentration and keeping the treatment system under alkaline condition were favorable to disposing of BaP-Cd combined pollution.Infrared scanning analysis showed that the structure of BaP changed with the action of microbe.Hydroxy,carbon-carbon double bond,aminoacyl and alkyl were confirmed to be the key functional groups for the strain to biodegrade BaP and adsorb Cd.