脂联素基因多态性与北方汉族人群缺血性脑卒中易感性关系研究

Study on the relationship between polymorphism of adiponectin gene and risk of ischemic stroke among Han population in the Northern parts of China

目的探讨脂联素基因多态性与北方汉族人群缺血性脑卒中易感性的关系。方法采用荧光定量PCR的TaqMan探针方法检测357例缺血性脑卒中首发病例及345名健康对照的脂联素基因（rs2241766、rs266729）多态性，用logistic回归分析计算各基因型与缺血性脑卒中的相关性。结果总体人群中rs2241766等位基因T〉G突变增加了缺血性脑卒中的易感性（OR=1．55，P=0．01）；调整各因素后，多因素logistic回归分析仍为缺血性脑卒中的危险因素（OR=1．55，P＝0．00）。rs266729多态性与总体人群缺血性脑卒中易感性无关（OR=1．13，P=0．57），但在女性中GG纯合突变增加了缺血性脑卒中的易感性（OR=3．25，P＝0．04）。结论rs2241766基因多态性与北方汉族总体人群缺血性脑卒中的易感性相关；rs266729等位基因GG纯合突变可能是北方汉族女性缺血性脑卒中易感性的危险闵素。

Objective To explore the association between polymorphisms of adiponectin gene and the risk of ischemic stroke in Han population from the Northern parts of China. Methods TaqMan probe of RT-PCR was applied to detect the genotype frequency of single nucleotide polymorphism （SNPs） （rs266729 and rs2241766） of adiponectin gene in 357 ischemic stroke cases who developed the episode at first time and with 345 healthy controls. Logistic regression analysis was used to evaluate the relationship of each genotype of SNPs and ischemic stroke. Results Mutation of rs2241766 （T〉G） increased the risk of ischemic stroke among all the samples （OR= 1.55, P=0.01 ） and it was still the risk factor of ischemic stroke when analyzed by multi-factors logistic regression after each factor was adjusted （OR=1.55, P=0.00）. The polymorphism of rs266729 was not related to the risk of ischemic stroke among all the samples （OR= 1.13, P=0.57）. However, the genotype GG of rs266729 increased the risk of ischemic stroke among female population （OR = 3.25, P= 0.04）. Conclusion The variance of rs2241766 in adiponectin gene was related to the risk of ischemic stroke in Han population from the Northern parts of China and the genotype GG of rs266729 could possibly increase the risk of ischemic stroke in women of Han population from the Northern parts of the country.