毛细管电泳-电化学发光法测定盐酸曲马多制剂及血浆中曲马多

Capillary Electrophoresis-Electrochemiluminescence Determination of Tramadol in Preparations of Tramadol Hydrochloride and Blood Plasma

采用毛细管电泳结合柱末电化学发光检测器提出了测定盐酸曲马多制剂及血浆中曲马多含量的方法。检测的原理系基于曲马多分子中的叔氨基对在pH9的磷酸盐缓冲介质中钌联吡啶络离子[Ru(Bpy)23+]与溶解氧在铂盘工作电极上反应的电化学发光的增强作用。对毛细管电泳及电化学发光检测的试验条件进行了优化。在优化的试验条件下,制剂中曲马多在1.0×10-8～7.0×10-6mol.L-1,血浆中曲马多在2.0×10-7～6.0×10-6mol.L-1范围内呈线性,检出限(3S/N)分别为7.0×10-9mol.L-1和1.0×10-7mol.L-1。采用该方法对盐酸曲马多制剂和血浆中曲马多的浓度分别进行了测定,所得平均回收率分别为98.9%和89.9%。

By using capillary electrophoresis in combination with the end-column electrochemiluminescence detection （CE-ECL） which is based on the chemiluminescence reaction between tris-（2,2′-bipyridine） ruthenium [Ru（bpy）3^2＋ ] and dissolved oxygen in PBS of pH 9 at the Pt-disc working electrode by the tert amino group present in the molecule of tramadol, a novel method for determination of tramadol in its preparations and in blood plasma was proposed. Optimum conditions of CE and ECL were studied and described in detail. Linearity ranges between 1.0 ×10^-8- 7. 0×10^-6 mol · L^-1 （for determination of tramadol in preparations） and 2. 0 ×10^-7 - 6. 0×10^-6 mol · L^-1 （for determination of tramadol in blood plasma） were obtained with detection limits （3S/N） of 7.0 ×10^-9mol · L^-1 and 1.0×10^-7mol · L^-1 respectively. Recovery and precision of the method were tested by standard addition method at 3 different concentration levels, the results of average recovery found were 98. 9% （for preparations） and 89. 9% （for blood plasm）.