摘要
取1mL口腔炎喷雾剂样品,用甲醇-水(1+1)溶液定量稀释至10mL,分取此溶液25μL供反相高效液相色谱法测定其绿原酸和咖啡酸含量。采用Shim-packVP-ODS色谱柱分离,以甲醇-0.012mol·L-1磷酸(27+73)混合溶液为流动相,柱温为30℃,流量为0.8mL·min-1,紫外检测器波长为323nm。结果表明:绿原酸在0.51～91.80mg·mL-1,咖啡酸在2.0～360.0mg·mL-1范围内呈线性关系,用标准加入法测定方法的回收率,结果依次在95.9%～97.4%和100.1%～100.9%范围内,检出限(3S/N)依次为0.10,0.40mg·mL-1,测定下限(10S/N)依次为0.36,1.40mg·mL-1。
One mL of the stomatitis curing sprays was taken and diluted with CH3 OH-H2 O（ 1 ＋1） to 10 mL in a brown glass volumetric flask An aliquot of 25 μL was taken for RP-HPLC determination of chlorogenic acid and caffeic acid. Shim-pack VP-ODS chromatographic column was used for separation and a mixed solution of methanol and 0. 012 mol · L^-1 H3PO4 （27＋73） was used as mobile phase. Column temperature of 30 ℃, flow-rate of 0. 8 mL · min^-1 and UV-detection at 323 nm were adopted in the analysis. Linear relationships between values of peak area and mass concentration of chlorogenic acid and caffeic acid were obtained in the ranges of 0. 51- 91.80 mg · L^-1 and 2. 0 - 360. 0 mg · L^-1 respectively. Values of detection limit （3S/N） and lower limit of determination （10S/N） found were 0. 10, 0. 40 mg · L^-1 and 0. 36, 1.40 mg · L^-1 respectively. Tests for recovery were made and the results obtained were in the ranges of 95. 9%- 97. 4% （for chlorogenic acid） and 100. 1%- 100. 9% （for caffeic acid）.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2010年第2期172-174,177,共4页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
重庆市自然科学基金项目(CSTC-2007BB0367)
