葡萄糖和蛋白胨对绿色木霉纤维素酶系分泌特性的影响

Effects of Glucose and Peptone on Characteristics of Cellulase System Induced by Trichoderma Viride

采用正交试验法分析出绿色木霉3.3711液体发酵产纤维素酶系各组分的最适酶解条件,其组合分别为C1（pH 5.0、45℃）,Cx（pH 3.5、55℃）和βG（pH 4.5、65℃）。在液体基础产酶培养基发酵条件下,三种组分酶的最高酶活分别为53.6、185.2、79.3 u/mL,比酶活分别为46.6、167.7、71.8 u/mg,最高的酶活形成时间分别为9、10、10 d。添加质量分数0.0005~0.003的葡萄糖,三种组分酶最高酶活分别降低了27.4%~38.4%、25.5%~35.5%、14.0%~52.3%,C1和Cx的最高酶活形成时间分别缩短为4~7、5 d,C1和βG比酶活分别降低了20.8%~35.4%、25.8%~64.5%;添加质量分数0.0005~0.01的蛋白胨,提高了C1和Cx最高酶活（分别为0.9%~199.6%、2.1%~45.1%）,缩短了C1和Cx的最高酶活形成时间（分别为4~7、4 d）,降低了βG的最高酶活（5.9%~34.2%）和比酶活（35.2%~87.3%）,对C1和Cx比酶活的增减效应存在浓度依赖性,质量分数0.0005时分别提高50.0%、65.4%。两种添加物均能明显改变C1和Cx的分泌变化规律,对βG酶影响极小。

By adopting orthogonal test,the optimal enzymolysis condition for respective components of cellulase system from Trichoderma viride 3.3711 induced liquid fermentation are found to be pH 5.0,45 ℃ for enzyme C1,pH 3.5,65 ℃ for enzyme Cx,and pH 4.5,65 ℃ for enzyme βG.Under the condition of liquid state and basic cultivation fermentation,the highest enzyme activity（HEA） of the three components are 53.6,185.2 and 79.3 u/mL,enzyme activity ratio（EAR） are 46.6,167.7 and 71.8 u/mg,formation time of highest enzyme activity（FTHEA） are 9,10 and 10 d,respectively.Addition of 0.05%～0.3% glucose leads to decreasing HEA of the three components by 27.4%～38.4%,25.5%～35.5% and 14.0%～52.3%,shortening FTHEA of C1 and Cx to 4～7 and 5 d,and decreasing EAR of C1 and βG by 20.8%～35.4% and 25.8%～64.5%,respectively.Addition of 0.05%～1.0% peptone leads to increasing HEA of C1 and Cx by 0.9%～199.6% and 2.1%～45.1%,shortening FTHEA of C1 and Cx to 4～7 and 4 d,and decreasing HEA and EAR of βG by 5.9%～34.2% and 35.2%～87.3%,respectively.The effects of peptone on EAR of C1 and Cx are concentration dependent and concentration 0.05% leads to increasing the effects by 50.0% and 65.4%,respectively.Regarding to the rule of enzyme secretion of respective components,glucose and peptone both show a certain effect on C1 and Cx and few effect on βG.