大豆胰蛋白酶抑制因子对獭兔胰腺组织学结构的影响

Effects of Soybean Trypsin Inhibitors on Histological Structure of Pancreas in Rex Rabbits

选择（42±4）日龄、平均体重为（711.4±108.8）g的健康美系獭兔断奶幼兔30只,公母比例为1∶1,根据性别、体重,将30只兔随机分为3组,每组10只,公母各半,单笼饲养。3个组日粮为等能等蛋白质设计,日粮中分别含有25%生大豆、25%化学钝化大豆、25%豆粕。胰蛋白酶抑制因子含量（实测值）分别为6.55、1.99和0.81 mg/g。试验6周后,从每一个组中随机选出3只公兔进行屠宰（（91±4）日龄、平均体重为1544.2 g±114.8 g）,宰前空腹46 h,取胰腺样品在10%中性甲醛液中固定,常规石蜡切片,HE染色,用于光学显微镜组织学观察。电子显微镜观察样品用2.5%戊二醛固定,常规树脂包埋,超薄切片,铀—铅染色。光学显微镜对胰腺组织结构观察结果表明,25%生大豆组幼兔胰腺间纤维性结缔组织增生,叶间水肿,局部腺组织消失,细胞水肿,边界模糊。电子显微镜对胰腺细胞观察结果表明,生大豆组幼兔的胰腺细胞核不规则,线粒体水肿,嵴消失,局部膜破裂,内质网减少,分泌颗粒极少或消失。25%生大豆组獭兔胰腺组织结构发生异常变化,而豆粕组和化学钝化大豆组正常。

30 healthy（42±4） day-old weaned rex rabbits with the average initial body weight of（711.4±108.8） g were randomly allocated to 3 groups with 5 males and 5 females each group.All rabbits were raised in separated cages.The diets for each group contained same level of crude protein and energy.The diets contained 25% raw soybean,25% chemically-processed soybean and 25% soybean-meal.The TI concentration（mensurated value） was 6.55,1.99 and 0.81 mg/g respectively.The experiment lasted for 6 weeks,and at the end of the experiment 3 male rabbits randomly chosen from each group were slaughtered（（91±4） day old,average body weight of 1544.2 g±114.8 g））after 4 to 6 hours of fast to take pancreas samples.These samples were fixed in 10% neutral formaldehyde solution,using conventional paraffin slice and HE dye for optical microscope observation.Samples for electronic microscope observation were fixed in 2.5% glutaraldehyde,conventionally resin embedded,very thin sliced,and uranium-plumbum dyed.Optical microscope observation showed the hyperplasia of fibroid connective tissue among the pancreas,oedema between lobes,disappearance of part of the gland,cell oedema,and border illegibility of the rabbits fed with 25% raw soybean.Abnormity of pancreas karyon,chondriosome oedema,disappearance of crista,partial membrane rupture,decrease of endoplasmic reticulum,and significantly cutting down or vanishing of particle secretion were discovered by electronical microscope observation in rabbits fed by 25%raw soybean diet.25% raw soybean did not significantly increase the weight of pancreas,but abnormal changes occurred in histological structures.As for the other 2 groups,the results remained the same that no abnormal changes were found in histological structures.