摘要
克隆猪繁殖与呼吸综合征病毒河南分离株的ORF4基因,将疏水序列缺失后,再克隆到原核表达载体pET-32a中,得到阳性重组质粒,经IPTG诱导,纯化后由Western blotting分析,并将所得的包涵体、变性蛋白和复性蛋白分别免疫小鼠,获取的血清抗体进行效价和中和活性的检测。为深入研究GP4蛋白的免疫特征奠定了基础。
ORF4 gene of PRRSV Henan strain was cloned.By deletion hydrophobic sequence and cloned into the prokaryotic expression vector pET-32a,positive recombinant was successfully obtained with the induction of IPTG.Analyze purificated protein by western blotting.Immunifaction thought cytorrhyctes,degenerated protein,reannealing protein.Detect titer and neutralization of the blood serum.It can be used for the further investigation on immunogenicity of GP4 protein of PRRSV.
出处
《中国畜牧兽医》
CAS
北大核心
2010年第1期57-61,共5页
China Animal Husbandry & Veterinary Medicine
基金
河南省重点攻关项目(082301180)
