耐碳青霉烯类鲍曼不动杆菌分子流行病学研究

Investigation on Epidemiology of Carbapenm-Resistant Acinetobacer Baumannii

目的探讨北京大学某三甲医院2007年3月-2008年3月显著增多的耐碳青霉烯类鲍曼不动杆菌之间的同源性，了解是否有该耐药株暴发流行。方法用脉冲场凝胶电泳对49株耐碳青霉烯类鲍曼不动杆菌进行分型，明确其是否为同一菌株的克隆。结果49株耐碳青霉烯类鲍曼不动杆菌分为Ⅰ，Ⅱ，Ⅲ，Ⅳ，Ⅴ，Ⅵ，Ⅶ七型，其中Ⅰ，Ⅱ，Ⅲ型为主要的流行型别。结论该院耐碳青霉烯类鲍曼不动杆菌存在克隆播散。

Objective To learn an outbreak of Acinetobacer baumannii by investigating the homology of the unusually increasing carbapenem-resistant Acinetobacer baumannii （CRAB） isolates selected March 2007 to March 2008 in atop three hospial in Beijing University. Methods The pulse-field gel electrophoresis （PFGE） was used to type 49 CRAB isolates gathered in the hospital,determing whether they derived from the same clone or not. Results 49 strains were classified into 7 types based on PFGE profiles,named type Ⅰ,Ⅱ,Ⅲ,Ⅳ,Ⅴ,Ⅵ and Ⅶ.There were 3 genotypes （Ⅰ,Ⅱ,Ⅲ ） mainly. Conclusion There has been clonal spread of CRAB strains among patients in the hospital.